A0793
Anti-Dog IgG (whole molecule)–Alkaline Phosphatase antibody produced in rabbit
IgG fraction of antiserum, buffered aqueous solution
Synonym(s):
Rabbit Anti-Dog IgG (whole molecule)–AP
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About This Item
Conjugate:
alkaline phosphatase conjugate
Clone:
polyclonal
Application:
DB, ELISA (d), WB CL
Citations:
8
biological source
rabbit
conjugate
alkaline phosphatase conjugate
antibody form
IgG fraction of antiserum
antibody product type
secondary antibodies
clone
polyclonal
form
buffered aqueous solution
species reactivity
dog
technique(s)
direct ELISA: 1:6,000, dot blot: 1:4,000, western blot (chemiluminescent): 1:4,000
shipped in
wet ice
storage temp.
2-8°C
target post-translational modification
unmodified
Quality Level
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General description
An immunoglobulin has two heavy chain and two light chain connected by disulfide bond. It mainly helps in immune defense. It is a glycoprotein. IgG is a major class of immunoglobulin. In dogs four subclasses of IgG (IgG1 -IgG4) are present.
Dog IgGs are glycoprotein antibodies that regulate several immunological responses. Canine IgGs can be used as markers for detecting immune-mediated haemolytic anaemia, adverse food reactions and also for assessing the risk of L. infantum transmission in dogs.
Immunogen
IgG isolated from pooled normal dog serum
Application
Anti-Dog IgG (whole molecule)−Alkaline Phosphatase antibody produced in rabbit has been used in enzyme-linked immunosorbent assay (ELISA), and immunoblotting.
Biochem/physiol Actions
Anti-Dog IgG (whole molecule) antibody is specific for IgG in dogs when tested against normal dog serum and dog IgG by double-diffusion and immunoelectrophoretic assays prior to conjugation.
Immunoglobulin G (IgG) participates in hypersensitivity type II and type III.
Physical form
Solution in 0.05 M Tris, pH 8.0, containing 1% bovine serum albumin, 1 mM MgCl2 and 15 mM sodium azide
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Storage Class
10 - Combustible liquids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
Regulatory Information
常规特殊物品
低风险生物材料
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C Sriwichai et al.
The Journal of small animal practice, 52(9), 476-483 (2011-09-08)
Canine allo- or autoantibodies are clinically important, but attachment of these immunoglobulin G (IgG) antibodies does not produce observable haemagglutination. Antibody to canine globulins is required to demonstrate sensitisation of red blood cells. Commercial reagents are available, but these often
Delivery of recombinant gene product to canine brain with the use of microencapsulation
Barsoum SC, et al.
The Journal of Laboratory and Clinical Medicine, 142(6), 399-413 (2003)
Joseph A Impellizeri et al.
Journal of translational medicine, 16(1), 349-349 (2018-12-13)
we have recently shown that Tel-eVax, a genetic vaccine targeting dog telomerase (dTERT) and based on Adenovirus (Ad)/DNA Electro-Gene-Transfer (DNA-EGT) technology can induce strong immune response and increase overall survival (OS) of dogs affected by multicentric Diffuse Large B cell
A Bead-Based Flow Cytometric Assay for Monitoring Yersinia pestis Exposure in Wildlife
Chandler JC, et al.
Journal of Clinical Microbiology, 56(7) (2018)
Comparative evaluation of several methods for Canine Visceral Leishmaniasis diagnosis
Silva DT da, et al.
Revista Brasileira de Parasitologia veterinaria = Brazilian Journal of Veterinary Parasitology : Org?o Oficial do Colegio Brasileiro de Parasitologia Veterinaria, 23(2), 179-186 (2014)
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