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Merck
CN

A1233

AMPK (α1/β1/γ1), active, His tagged human

PRECISIO® Kinase, recombinant, expressed in baculovirus infected Sf9 cells, ≥70% (SDS-PAGE), buffered aqueous glycerol solution

Synonym(s):

AMPKG, HAMPKb, MGC17785, MGC33776, MGC57364, MGC8666, PRKAA1, PRKAB1, PRKAG1

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About This Item

NACRES:
NA.32
UNSPSC Code:
12352200

Key Documents

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Product Name

AMPK (α1/β1/γ1), active, His tagged human, PRECISIO® Kinase, recombinant, expressed in baculovirus infected Sf9 cells, ≥70% (SDS-PAGE), buffered aqueous glycerol solution

biological source

human

recombinant

expressed in baculovirus infected Sf9 cells

product line

PRECISIO® Kinase

assay

≥70% (SDS-PAGE)

form

buffered aqueous glycerol solution

specific activity

626-848 nmol/min·mg

mol wt

A1 subunit ~68 kDa
B1 subunit ~38 kDa
G1 subunit ~40 kDa

solubility

water: soluble

UniProt accession no.

P54619
Q13131
Q9Y478

application(s)

life science and biopharma

shipped in

dry ice

storage temp.

−70°C

Quality Level

200

Gene Information

human ... PRKAA1(5562), PRKAB1(5564), PRKAG1(5571)

Application

AMP-activated protein kinase (AMPK) has been used to access the binding kinetics of thalidezine to recombinant active human AMPK using Bio-Layer Interferometry (BLI) binding assay.

Biochem/physiol Actions

AMP-activated protein kinase (AMPK) is an important energy-sensing enzyme that monitors cellular energy status. In response to cellular metabolic stresses, AMPK is activated and phosphorylates and inactivates acetyl-CoA carboxylase (ACC) and 3-hydroxy-3-methylglutaryl-CoA reductase (HMGCR), key enzymes involved in regulating biosynthesis of fatty acid and cholesterol.

General description

Research area: CELL SIGNALING

AMP-activated protein kinase (AMPK) is a heterotrimer protein kinase consisting of a α catalytic subunit, and non-catalytic β and γ subunits.

Physical form

Supplied in 50mM NaPhosphate pH 7.0, 300 mM NaCl, 150 mM imidazole, 0.1mM PMSF, 0.2 mM DTT, 25% glycerol.

Legal Information

PRECISIO is a registered trademark of Merck KGaA, Darmstadt, Germany

pictograms

Health hazardExclamation mark
GHS08,GHS07

signalword

Danger

Hazard Classifications

Eye Irrit. 2 - Repr. 1B - Skin Irrit. 2

Storage Class

6.1C - Combustible acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects

wgk

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Faceshields, Gloves, Goggles, type ABEK (EN14387) respirator filter

Regulatory Information

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Certificates of Analysis (COA)

Lot/Batch Number
SLCR0336
SLBL3572V
SLBG1922V

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Thalidezine, a novel AMPK activator, eliminates apoptosis-resistant cancer cells through energy-mediated autophagic cell death
Law BTK
Oncotarget, 8(18), 30077?30091-30077?30091 (2017)
AMPK: guardian of metabolism and mitochondrial homeostasis
Herzig S and Shaw RJ
Nature Reviews in Molecular and Cell Biology, 19, 121?135 -121?135 (2018)
Yasuhiko Minokoshi et al.
Nature, 428(6982), 569-574 (2004-04-03)
Obesity is an epidemic in Western society, and causes rapidly accelerating rates of type 2 diabetes and cardiovascular disease. The evolutionarily conserved serine/threonine kinase, AMP-activated protein kinase (AMPK), functions as a 'fuel gauge' to monitor cellular energy status. We investigated
D G Hardie et al.
European journal of biochemistry, 246(2), 259-273 (1997-06-01)
A single entity, the AMP-activated protein kinase (AMPK), phosphorylates and regulates in vivo hydroxymethylglutaryl-CoA reductase and acetyl-CoA carboxylase (key regulatory enzymes of sterol synthesis and fatty acid synthesis, respectively), and probably many additional targets. The kinase is activated by high

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