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A1346

Sigma-Aldrich

ASB-14

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Synonym(s):
3-[N,N-Dimethyl(3-myristoylaminopropyl)ammonio]propanesulfonate, Amidosulfobetaine-14
Empirical Formula (Hill Notation):
C22H46N2O4S
CAS Number:
Molecular Weight:
434.68
MDL number:

description

zwitterionic

Quality Level

Assay

≥95.0% (HPLC)

form

powder

mol wt

average mol wt >10,000

aggregation number

≥23

storage temp.

2-8°C

InChI

1S/C22H46N2O4S/c1-4-5-6-7-8-9-10-11-12-13-14-17-22(25)23-18-15-19-24(2,3)20-16-21-29(26,27)28/h4-21H2,1-3H3,(H-,23,25,26,27,28)

InChI key

UTSXERRKRAEDOV-UHFFFAOYSA-N

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This Item
182750SAB1403263SAB1407509
ASB-14

Sigma-Aldrich

A1346

ASB-14

ASB-14 A zwitterionic amidosulfobetaine detergent useful for solubilizing proteins visualized by 2D-electrophoresis.

Millipore

182750

ASB-14

assay

≥95.0% (HPLC)

assay

≥97% (HPLC)

assay

-

assay

-

form

powder

form

solid

form

buffered aqueous solution

form

buffered aqueous solution

mol wt

average mol wt >10,000

mol wt

-

mol wt

antigen ~38.1 kDa

mol wt

antigen ~38.9 kDa

aggregation number

≥23

aggregation number

-

aggregation number

-

aggregation number

-

storage temp.

2-8°C

storage temp.

10-30°C

storage temp.

−20°C

storage temp.

−20°C

General description

ASB-14 comprises alkyl tails and amidosulfobetaine headgroup.

Application

ASB-14 has been used as a component of rehydration buffer for proteomic sample preparation from Leishmania donovani.
Zwitterionic detergent. Useful for solubilization of proteins, including membrane proteins, for 2D electrophoresis.

Biochem/physiol Actions

ASB-14 is useful in proteasome extraction especially for the membrane associated proteins. Along with CHAPS (3-(3-cholamidopropyl)dimethylammonio)-1-propanesulfonate), it is employed in protein solubilization even in the presence of thiourea.

Analysis Note

ASB-14 (amidosulfobetaine-14), a membrane solubilizing zwitterionic detergent (medium chain amidosulfobetaine surfactant), may be used in protein and macromolecule research for the preparative extraction and solubilization of proteins for separation and analysis by methods such as 2D-electrophoresis. ASB-14 may be used in tissue engineering for antigen removal (AR) for xenogeneic scaffold generation. ASB-14 may be used to develop and study zwitterionic micelles.

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

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