Skip to Content
Merck
CN

A1935

Photobiotin acetate salt

For labeling DNA probes for hybridizations

Synonym(s):

Biotin {3-[3-(4-azido-2-nitroanilino)-N-methylpropylamino]propylamide} acetate salt, N-(4-Azido-2-nitrophenyl)-N′-(3-biotinylaminopropyl)-N′-methyl-1,3-propanediamine acetate salt

Sign In to View Organizational & Contract Pricing.

Select a Size

About This Item

Empirical Formula (Hill Notation):
C23H35N9O4S · C2H4O2
CAS Number:
96087-38-6
Molecular Weight:
593.70
NACRES:
NA.52
PubChem Substance ID:
24890604
UNSPSC Code:
12352200
MDL number:
MFCD00210085

Key Documents

View All Documentation
Technical Service
Need help? Our team of experienced scientists is here for you.
Let Us Assist
Technical Service
Need help? Our team of experienced scientists is here for you.
Let Us Assist

Product Name

Photobiotin acetate salt, For labeling DNA probes for hybridizations

InChI

1S/C23H35N9O4S.C2H4O2/c1-31(12-4-10-25-17-9-8-16(29-30-24)14-19(17)32(35)36)13-5-11-26-21(33)7-3-2-6-20-22-18(15-37-20)27-23(34)28-22;1-2(3)4/h8-9,14,18,20,22,25H,2-7,10-13,15H2,1H3,(H,26,33)(H2,27,28,34);1H3,(H,3,4)/t18-,20-,22-;/m0./s1

SMILES string

CC(O)=O.CN(CCCNC(=O)CCCC[C@@H]1SCC2NC(=O)NC12)CCCNc3ccc(cc3[N+]([O-])=O)N=[N+]=[N-]

InChI key

FFBLNTOMOSLSQM-AYEYRVMASA-N

grade

Molecular Biology

assay

≥95%

form

film or powder

solubility

H2O: 10 mg/mL (Stable for at least 5 months if kept frozen and protected from light.)

storage temp.

−20°C

Quality Level

200

Looking for similar products? Visit Product Comparison Guide

Application

Photobiotin acetate salt has been used to label DNA probes to perform DNA−DNA hybridization to determine genomic relatedness.

Biochem/physiol Actions

Photobiotin acetate is capable of labeling single stranded DNA and linear or supercoiled double-stranded DNA.
Photobiotin is a photo-activatable analog of biotin used primarily for labeling DNA, RNA and proteins. Probes prepared using photobiotin can detect target DNA as little as 0.5 pg.

Disclaimer

Light sensitive.

General description

Photobiotin acetate is made up of biotin, which is bound with the help of a charged linker arm to a photoreactive arylazide group.

Storage Class

11 - Combustible Solids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)

Choose from one of the most recent versions:

Certificates of Analysis (COA)

Lot/Batch Number
0000427551
0000427551
0000387187
0000327749
0000325058

Don't see the Right Version?

If you require a particular version, you can look up a specific certificate by the Lot or Batch number.

Search for a COA

Already Own This Product?

Find documentation for the products that you have recently purchased in the Document Library.

Visit the Document Library

Preparation and uses of photobiotin
Test, 184, 588-600 (1990)
Bao-Zhu Fang et al.
Antonie van Leeuwenhoek, 112(6), 857-865 (2019-01-04)
A novel actinobacterial strain, designated YIM ART13T, was isolated from a soil sample collected from a karst cave in Xingyi county, Guizhou province, South western China. The taxonomic position of the strain was investigated using a polyphasic approach. Cells of
Chang Soo Lee et al.
International journal of systematic and evolutionary microbiology, 57(Pt 8), 1775-1779 (2007-08-09)
A novel thiosulfate-oxidizing bacterium, designated strain LCS2(T), was isolated from a biofilm on sulfur particles used in an autotrophic denitrification process. The strain was found to comprise Gram-negative, non-motile, non-spore-forming rods that produced yellow-pigmented colonies on R2A agar. The strain
Preparation and uses of photobiotin
Methods in Enzymology, 184, 588-600 (1990)
Rhodanobacter thiooxydans sp. nov., isolated from a biofilm on sulfur particles used in an autotrophic denitrification process
Lee CS, et al.
International Journal of Systematic and Evolutionary Microbiology, 57(8), 1775-1779 (2007)
View All Related Papers

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Contact Technical Service