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A1935

Photobiotin acetate salt

For labeling DNA probes for hybridizations

Synonym(s):

Biotin {3-[3-(4-azido-2-nitroanilino)-N-methylpropylamino]propylamide} acetate salt, N-(4-Azido-2-nitrophenyl)-N′-(3-biotinylaminopropyl)-N′-methyl-1,3-propanediamine acetate salt

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About This Item

Empirical Formula (Hill Notation):
C23H35N9O4S · C2H4O2
CAS Number:
96087-38-6
Molecular Weight:
593.70
NACRES:
NA.52
PubChem Substance ID:
24890604
UNSPSC Code:
12352200
MDL number:
MFCD00210085
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grade

Molecular Biology

Quality Level

200

assay

≥95%

form

film or powder

solubility

H2O: 10 mg/mL (Stable for at least 5 months if kept frozen and protected from light.)

storage temp.

−20°C

SMILES string

CC(O)=O.CN(CCCNC(=O)CCCC[C@@H]1SCC2NC(=O)NC12)CCCNc3ccc(cc3[N+]([O-])=O)N=[N+]=[N-]

InChI

1S/C23H35N9O4S.C2H4O2/c1-31(12-4-10-25-17-9-8-16(29-30-24)14-19(17)32(35)36)13-5-11-26-21(33)7-3-2-6-20-22-18(15-37-20)27-23(34)28-22;1-2(3)4/h8-9,14,18,20,22,25H,2-7,10-13,15H2,1H3,(H,26,33)(H2,27,28,34);1H3,(H,3,4)/t18-,20-,22-;/m0./s1

InChI key

FFBLNTOMOSLSQM-AYEYRVMASA-N

General description

Photobiotin acetate is made up of biotin, which is bound with the help of a charged linker arm to a photoreactive arylazide group.

Application

Photobiotin acetate salt has been used to label DNA probes to perform DNA−DNA hybridization to determine genomic relatedness.

Biochem/physiol Actions

Photobiotin acetate is capable of labeling single stranded DNA and linear or supercoiled double-stranded DNA.
Photobiotin is a photo-activatable analog of biotin used primarily for labeling DNA, RNA and proteins. Probes prepared using photobiotin can detect target DNA as little as 0.5 pg.

Disclaimer

Light sensitive.

Storage Class

11 - Combustible Solids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)

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Certificates of Analysis (COA)

Lot/Batch Number
0000427551
0000427551
0000387187
0000327749
0000325058

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Preparation and uses of photobiotin
Test, 184, 588-600 (1990)
Bao-Zhu Fang et al.
Antonie van Leeuwenhoek, 112(6), 857-865 (2019-01-04)
A novel actinobacterial strain, designated YIM ART13T, was isolated from a soil sample collected from a karst cave in Xingyi county, Guizhou province, South western China. The taxonomic position of the strain was investigated using a polyphasic approach. Cells of
Preparation and uses of photobiotin
Methods in Enzymology, 184, 588-600 (1990)
Chang Soo Lee et al.
International journal of systematic and evolutionary microbiology, 57(Pt 8), 1775-1779 (2007-08-09)
A novel thiosulfate-oxidizing bacterium, designated strain LCS2(T), was isolated from a biofilm on sulfur particles used in an autotrophic denitrification process. The strain was found to comprise Gram-negative, non-motile, non-spore-forming rods that produced yellow-pigmented colonies on R2A agar. The strain
H Zreiqat et al.
Molecular biotechnology, 10(2), 107-113 (1998-11-20)
The universal quantitation of the DNA hybridization reaction has been a goal sought by many researchers. Part of this search has been the need to develop a rapid, sensitive, easy-to-perform, and quantitative method to measure the abundance of specific mRNAs
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Global Trade Item Number

SKUGTIN
A1935-1MG04061833343210
A1935-2MG04061833343227

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