A1949
Anti-Rabbit IgG (γ-chain specific)–Peroxidase antibody, Mouse monoclonal
clone RG-96, purified from hybridoma cell culture
Synonym(s):
Monoclonal Anti-Rabbit IgG (γ-chain specific)
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About This Item
NACRES:
NA.46
UNSPSC Code:
12352203
Conjugate:
peroxidase conjugate
Clone:
RG-96, monoclonal
Application:
ELISA (d), IHC (p), WB
Citations:
86
biological source
mouse
Quality Segment
conjugate
peroxidase conjugate
antibody form
purified immunoglobulin
antibody product type
secondary antibodies
clone
RG-96, monoclonal
form
lyophilized powder
species reactivity
rabbit
should not react with
goat, feline, pig, guinea pig, rat, bovine, canine, human, horse, sheep, chicken
packaging
vial of 0.5 mL
technique(s)
direct ELISA: 1:40,000, immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:200, western blot: 1:160,000 using total cell extract of HeLa cells (5-10 μg/well)
isotype
IgG1
storage temp.
2-8°C
target post-translational modification
unmodified
General description
Anti-Rabbit IgG (γ-chain specific)-Peroxidase antibody, Mouse monoclonal is a monoclonal anti-rabbit IgG conjugated to horseradish peroxidase. Monoclonal antirabbit IgG is derived from the RG-96 hybridoma produced by the fusion of mouse myeloma cells and splenocytes from mice immunized with rabbit IgG.
Rabbit IgGs are often used as primary antibodies in several immunological assays. Thus, polyclonal anti-rabbit IgGs are usually used as secondary antibodies in such applications. However, polyclonal antibodies to rabbit IgGs can also cross-react with IgGs from other species, especially with human IgGs, and thereby lead to a lack of specificity in test results. The use of monoclonal anti-rabbit IgG that is specific for only rabbit IgG improves the accuracy of immunological assays and thus, can be useful for various research applications.
The product recognizes an epitope located on the γ (heavy)-chain of rabbit IgG. In immunoblotting, the antibody recognizes both native and denatured forms of rabbit IgG. In ELISA, the antibody is specific for rabbit IgG, and shows no cross-reactivity with rabbit IgA and IgM or human IgG, IgA, and IgM. No cross reaction is observed with IgG from the following species: bovine, cat, chicken, dog, goat, guinea pig, horse, pig, rat, or sheep.
Immunogen
Rabbit IgG
Application
Monoclonal Anti-Rabbit IgG (γ-chain specific)- Peroxidase has been used in
- various immunochemical assays like enzyme linked immunosorbent assay (ELISA)
- westernblotting
- immunohistology
- dot immunobinding assay
Physical form
Lyophilized from 0.01 M phosphate buffered saline, pH 7.4, containing 1% bovine serum albumin and 0.05% MIT.
Preparation Note
Prepared by the two-step glutaraldehyde method described by Avrameas, S., et al., Scand. J. Immunol., 8, Suppl. 7, 7 (1978).
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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signalword
Warning
hcodes
Hazard Classifications
Skin Sens. 1
Storage Class
13 - Non Combustible Solids
wgk
WGK 2
flash_point_f
Not applicable
flash_point_c
Not applicable
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