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Sigma-Aldrich

ACCUSPIN™ Tubes Sterile, 50 mL Capacity

radiation sterilized tube fitted with a high density polyethylene barrier

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NACRES:
NA.75

Quality Level

200

sterility

sterile; irradiated

application(s)

hematology
histology

storage temp.

15-25°C

Related Categories

General description

ACCUSPIN™ Tubes are polypropylene radiation sterilized tube fitted with a high density polyethylene barrier. Each tube will accept 15 mL of the density gradient.

Application

ACCUSPIN™ Tubes Sterile, 50 mL Capacity is intended for use with the density gradient separation medium Histopaque™ -1077:


  • in the isolation of lymphocytes and other mononuclear cells.
  • isolation of cord blood–derived endothelial progenitor cells.
  • isolate mononuclear cell layer from bone marrow samples.
  • suitable for the purification of human bone marrow aspirates for the investigation of the therapeutic value of BET inhibitors for acute myeloid leukemia.

Principle

Following the addition of Histopaque-1077 to theACCUSPIN Tube, a brief centrifugation places theHistopaque-1077 below the frit. The blood sample canbe added to the top chamber of the tube without risk ofmixing with the Histopaque-1077 in the lower chamberunder the frit. On subsequent centrifugation the wholeblood descends through the frit to contact with theHistopaque-1077. The elements of greater densitydisplace a volume of Histopaque-1077 above the fritgiving a clear separation of the blood components. Theerythrocytes aggregate and the granulocytes becomeslightly hypertonic, increasing their sedimentation rate,resulting in pelleting at the bottom of the ACCUSPINTube. Lymphocytes and other mononuclear cells, e.g.,monocytes, remain at the plasma/Histopaque-1077interface. This dense band of mononuclear cells maybe collected by pouring off the contents of the upperchamber or by means of a pipette. Erythrocytecontamination is avoided due to the barrier between thechambers.

Quantity

Package contains 10 tubes

Legal Information

Accuspin is a trademark of Sigma-Aldrich Co. LLC

Pictograms

Health hazard
GHS08

Signal Word

Danger

Hazard Statements

H317 - H334

Hazard Classifications

Resp. Sens. 1 - Skin Sens. 1

Storage Class Code

12 - Non Combustible Liquids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

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Kara S Cox et al.
mAbs, 8(1), 129-140 (2015-10-23)
Monitoring antigen-specific memory B cells and the antibodies they encode is important for understanding the specificity, breadth and duration of immune response to an infection or vaccination. The antibodies isolated could further help design vaccine antigens for raising relevant protective
S A Mireku et al.
Scientific reports, 7(1), 14296-14296 (2017-11-01)
Bacterial ABC importers catalyze the uptake of essential nutrients including transition metals and metal-containing co-factors. Recently, an IgG antibody targeting the external binding protein of the Staphylococcus aureus Mn(II) ABC importer was reported to inhibit transport activity and reduce bacterial
Aimin Tang et al.
Nature communications, 10(1), 4153-4153 (2019-09-14)
Respiratory syncytial virus (RSV) infection is the leading cause of hospitalization and infant mortality under six months of age worldwide; therefore, the prevention of RSV infection in all infants represents a significant unmet medical need. Here we report the isolation
Juan M Melero-Martin et al.
Methods in enzymology, 445, 303-329 (2008-11-22)
Rapid and complete vascularization of ischemic tissues and thick engineered tissues is likely to require vasculogenesis. Therefore, the search for clinically relevant sources of vasculogenic cells and the subsequent development of experimental models of vasculogenesis is of utmost importance. Here
Samuel J S Rubin et al.
Nature communications, 10(1), 2686-2686 (2019-06-21)
Inflammatory bowel disease (IBD) includes Crohn's disease and ulcerative colitis. Each disease is characterized by a diverse set of potential manifestations, which determine patients' disease phenotype. Current understanding of phenotype determinants is limited, despite increasing prevalence and healthcare costs. Diagnosis
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