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A2220

ANTI-FLAG^® M2 Affinity Gel

Name: ANTI-FLAG<SUP>®</SUP> M2 Affinity Gel
Brand: SIGMA

purified immunoglobulin, buffered aqueous glycerol solution

Synonym(s):

Monoclonal ANTI-FLAG^® M2, ANTI-FLAG^® M2 Affinity Agarose Gel, Anti-ddddk, Anti-dykddddk

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About This Item

NACRES:

NA.32

UNSPSC Code:

12352203

Conjugate:

agarose conjugate

Clone:

M2, monoclonal

Application:

IP, affinity chromatography

Citations:

2532

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Quality Level

200

conjugate

agarose conjugate

antibody form

purified immunoglobulin

antibody product type

primary antibodies

clone

M2, monoclonal

form

buffered aqueous glycerol solution

analyte chemical class(es)

proteins

technique(s)

affinity chromatography: suitable, immunoprecipitation (IP): suitable

matrix

(4% agarose bead; 45-165μm bead size)

isotype

IgG1

capacity

>0.6 mg/mL, resin binding capacity (FLAG-BAP)

shipped in

wet ice

storage temp.

−20°C

Related Categories

FLAG Purification

Protein Purification Chromatography

Protein Sample Prep

Recombinant/Fusion Tag Protein Purification

General description

Anti-FLAG M2 Affinity gel is a mouse monoclonal antibody that is covalently attached to agarose. The antibody binds FLAG at the N-terminal, Met-N-terminal, C-terminal and internal locations of fusion proteins. Binding is calcium-independent.

Elution - FLAG^® peptide, Glycine, pH 3.5, 3x FLAG^® peptide

FLAG^® peptide, Glycine, pH3.5, 3x FLAG^® peptide

Immunogen

DYKDDDDK

Application

Anti-FLAG^® M2 affinity gel has been used for western blotting, immunoprecipitation and for the purification of FLAG fusion proteins.

Learn more product details in our FLAG^® application portal.

Physical form

Suspension in buffered saline containing azide as preservative and 50% glycerol

Other Notes

2024 CiteAb Award Winner for Supplier Succeeding in Parkinson′s Research

Legal Information

ANTI-FLAG is a registered trademark of Merck KGaA, Darmstadt, Germany

FLAG is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

FLAG^® tag, 3x FLAG^®, DYKDDDDK tag

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Storage Class

10 - Combustible liquids

flash_point_f

Not applicable

flash_point_c

Not applicable

Regulatory Information

常规特殊物品

低风险生物材料

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Stability of plant immune-receptor resistance proteins is controlled by SKP1-CULLIN1-F-box (SCF)-mediated protein degradation.

Yu Ti Cheng et al.

Proceedings of the National Academy of Sciences of the United States of America, 108(35), 14694-14699 (2011-08-30)

The nucleotide-binding domain and leucine-rich repeats containing proteins (NLRs) serve as immune receptors in both plants and animals. Overaccumulation of NLRs often leads to autoimmune responses, suggesting that the levels of these immune receptors must be tightly controlled. However, the

Ca2+/Calmodulin-dependent protein kinase kinase beta is regulated by multisite phosphorylation.

Michelle F Green et al.

The Journal of biological chemistry, 286(32), 28066-28079 (2011-06-15)

Ca(2+)/calmodulin-dependent protein kinase kinase β (CaMKKβ) is a serine/threonine-directed kinase that is activated following increases in intracellular Ca(2+). CaMKKβ activates Ca(2+)/calmodulin-dependent protein kinase I, Ca(2+)/calmodulin-dependent protein kinase IV, and the AMP-dependent protein kinase in a number of physiological pathways, including

Tandem affinity purification of miRNA target mRNAs (TAP-Tar).

Nora Nonne et al.

Nucleic acids research, 38(4), e20-e20 (2009-12-04)

MicroRNAs (miRNAs) bind to Argonaute proteins, and together they form the RISC complex and regulate target mRNA translation and/or stability. Identification of mRNA targets is key to deciphering the physiological functions and mode of action of miRNAs. In mammals, miRNAs

Integrative genomics identifies MCU as an essential component of the mitochondrial calcium uniporter.

Joshua M Baughman et al.

Nature, 476(7360), 341-345 (2011-06-21)

Mitochondria from diverse organisms are capable of transporting large amounts of Ca(2+) via a ruthenium-red-sensitive, membrane-potential-dependent mechanism called the uniporter. Although the uniporter's biophysical properties have been studied extensively, its molecular composition remains elusive. We recently used comparative proteomics to

The canonical NF-kappaB pathway governs mammary tumorigenesis in transgenic mice and tumor stem cell expansion.

Manran Liu et al.

Cancer research, 70(24), 10464-10473 (2010-12-17)

The role of mammary epithelial cell (MEC) NF-κB in tumor progression in vivo is unknown, as murine NF-κB components and kinases either are required for murine survival or interfere with normal mammary gland development. As NF-κB inhibitors block both tumor-associated

View All Related Papers

Articles

ANTI-FLAG^® M2 Magnetic Beads

The FLAG® Expression System is a proven method to express, purify and detect recombinant fusion proteins. Sigma®, the proven provider of FLAG®, now offers a magnetic bead for immunoprecipitation, protein purification, and the study of protein-protein interactions. The ANTI-FLAG® M2 Magnetic Bead is composed of murine derived, anti-FLAG® M2 monoclonal antibody attached to superparamagnetic iron impregated 4% agarose beads, with an average diameter of 50 µm. The M2 antibody is capable of binding to fusion proteins containing a FLAG peptide sequence at the N-terminus, Met-N-terminus, or C-terminus locations in mammalian, bacterial, and plant extracts.

ANTI-FLAG^® M2磁珠

FLAG®表达系统是一种表达、纯化和检测重组融合蛋白的成熟方法。作为FLAG®的可靠供应商，Sigma®现提供FLAG标签抗体标记的免疫磁珠，用于免疫沉淀、蛋白纯化和蛋白互作研究。ANTI-FLAG® M2磁珠由ANTI-FLAG® M2鼠单克隆抗体与超顺磁铁包覆的4%琼脂糖微珠偶联而成，平均粒径50 µM。M2抗体可结合哺乳动物、细菌和植物提取物融合蛋白N端、Met-N端或C端的FLAG®肽。

Protocols

Immunoprecipitation of FLAG Fusion Proteins Using Monoclonal Antibody Affinity Gels

Protocol for immunoprecipitation (IP) of FLAG fusion proteins using M2 monoclonal antibody 4% agarose affinity gels

采用单克隆抗体亲和凝胶进行的FLAG融合蛋白免疫沉淀_蛋白纯化-默克生命科学

免疫沉淀（IP）可用于高效、高产量分离和纯化与FLAG®肽标签融合的蛋白质。此过程采用ANTI-FLAG® M2亲和凝胶进行，后者是一种与琼脂糖树脂共价结合的高度特异性单克隆抗体。

Global Trade Item Number

SKU	GTIN
A2220-2X25ML	04061838089267
A2220-5ML	04061838251282
A2220-10ML	04061838061843
A2220-4X25ML	04061838251275
A2220-25ML	04061838251268
A2220-1ML	04061838061850

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