A2264
β-N-Acetylglucosaminidase from Canavalia ensiformis (Jack bean)
ammonium sulfate suspension, ≥10 units/mg protein
Synonym(s):
β-N-Acetyl-D-hexosaminide N-acetylhexosaminohydrolase, β-N-Acetylhexosaminidase
biological source
Canavalia ensiformis
Quality Level
form
ammonium sulfate suspension
specific activity
≥10 units/mg protein
foreign activity
α- and β-galactosidase, and α-L-fucosidase ≤0.1%
α-mannosidase ≤0.2%
storage temp.
2-8°C
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Application
β -N-acetylglucosaminidase is a lysosomal enzyme used to hydrolyze N-acetyl-β-D-glucosaminides and N-acetyl-β-Dgalactosaminides. It is used in chemoenzymatic synthesis of oligosaccharides based on their effective transglycosylation of β-GlcNAc and β-GalNAcc. It may be a useful tool to study Alzheimer′s Disease . Acetylglucosaminidase from Canavalia ensiformis has been used to study enzymic detachment of biofilms .
Biochem/physiol Actions
This enzyme, sometimes called β-N-acetylhexosaminidase, is reported to liberate terminal β-linked N-acetylglucosamine and N-acetylgalactosamine from a variety of substrates.
This enzyme, sometimes called β-N-acetylhexosaminidase, is reported to liberate terminal β-linked N-acetylglucosamine and N-acetylgalactosamine from a variety of substrates. The activity of β-N-actylglucosaminidase may be determined with the chromogenic substrate p-nitrophenyl-N-acetyl-β-D-glucosaminide. β-N-actylglucosaminidase hydrolyzes the terminal nonreducing N-acetyl-D-hexosamine residues. This enzyme contains two predominant isozymes, Hex A, a heterodimer, and Hex B, a homodimer. N-acetylglucosamine, acetamide, N-2-acetamido-2-deoyglucosylamine, N-acetylnojirimycin, and N-acetyldeoxynojirmycin are known inhibitors.
Physical form
Suspension in 2.5 M (NH4)2SO4, pH 7.0
Analysis Note
At pH 4.0, p-nitrophenyl β-N-acetylgalactosaminide is hydrolyzed at approximately 50% of the rate of hydrolysis of p-nitrophenyl β-N-acetylglucosaminide at pH 5.0.
Other Notes
One unit will hydrolyze 1.0 μmole of p-nitrophenyl N-acetyl-β-D-glucosaminide to p-nitrophenol and N-acetyl-D-glucosamine per min at pH 5.0 at 25 °C.
Storage Class Code
12 - Non Combustible Liquids
WGK
WGK 3
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Regulatory Information
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T Berger et al.
Journal of reproduction and fertility, 86(2), 559-565 (1989-07-01)
An assay to determine the binding of pig spermatozoa to the zona pellucida (ZP) of pig oocytes was developed using conditions compatible with in-vitro fertilization of pig eggs and with pig sperm penetration of zona-free hamster ova. These conditions were
Mohammed A Al-Fattani et al.
Journal of medical microbiology, 55(Pt 8), 999-1008 (2006-07-20)
Matrix material was extracted from biofilms of Candida albicans and Candida tropicalis and analysed chemically. Both preparations contained carbohydrate, protein, hexosamine, phosphorus and uronic acid. However, the major component in C. albicans matrix was glucose (32%), whereas in C. tropicalis
Chaeyoung Kim et al.
Neurobiology of aging, 34(1), 275-285 (2012-04-17)
Deposition of β-amyloid (Aβ) as senile plaques and disrupted glucose metabolism are two main characteristics of Alzheimer's disease (AD). It is unknown, however, how these two processes are related in AD. Here we examined the relationship between O-GlcNAcylation, which is
Benjamin Pluvinage et al.
Journal of molecular biology, 425(2), 334-349 (2012-11-17)
Streptococcus pneumoniae is a serious human pathogen that presents on its surface numerous proteins involved in the host-bacterium interaction. The carbohydrate-active enzymes are particularly well represented among these surface proteins, and many of these are known virulence factors, highlighting the
A M Dygai et al.
Bulletin of experimental biology and medicine, 153(5), 684-688 (2012-11-01)
Pegylated hyaluronate-endo-β-N-acetylhexosaminidase considerably potentiates the hemostimulating effects of erythropoietin due to intensification of proliferation and differentiation of erythroid precursors against the background of enhanced secretion of hemopoietins by nonadherent hemopoiesis-inducing environment cells and elevation of serum erythropoietin concentration. The use
Articles
Instructions for working with enzymes supplied as ammonium sulfate suspensions
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