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Merck
CN

A2278

N-Acetyl-D-glucosamine–Agarose

saline suspension

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About This Item

NACRES:
NA.56
PubChem Substance ID:
UNSPSC Code:
41106500
MDL number:
Technical Service
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form

saline suspension

Quality Level

matrix

6% beaded agarose

matrix activation

divinyl sulfone

matrix attachment

hydroxyl

matrix spacer

5 atoms

capacity

≥25 mg/mL binding capacity (Triticum vulgaris lectin)

storage temp.

2-8°C

SMILES string

CC(=O)N[C@@H](C=O)[C@@H](O)[C@H](O)[C@H](O)CO

InChI

1S/C8H15NO6/c1-4(12)9-5(2-10)7(14)8(15)6(13)3-11/h2,5-8,11,13-15H,3H2,1H3,(H,9,12)/t5-,6+,7+,8+/m0/s1

InChI key

MBLBDJOUHNCFQT-LXGUWJNJSA-N

General description

N-Acetyl-D-glucosamine is used in affinity chromatography, protein chromatography, and carbohydrate matrices. N-Acetyl-D-glucosamine can be used to purify wheat germ (Triticum vulgaris) lectin.

Application

N-Acetyl-D-glucosamine-Agarose has been used

  • for the incubation of supernatant for the depletion of ficolin-3-deficient serum
  • for detecting the lectin activity by ligand binding assays
  • to produce ficolin 2 depleted serum

Physical form

Suspension in 0.15 M NaCl, 0.01 M sodium phosphate, pH 6.8, containing 0.02% sodium azide


Storage Class

10 - Combustible liquids

wgk

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable



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R Sullivan et al.
Biochemical and biophysical research communications, 162(1), 184-188 (1989-07-14)
Human ejaculated spermatozoa are heterogeneous and can be separated into two distinct populations according to their respective buoyant densities. In order to investigate the functional differences between these two types of spermatozoa, we have searched for the presence of galactosyltransferase.
M B Allen et al.
The Biochemical journal, 185(3), 565-575 (1980-03-01)
1. Procedures for the extensive purification in high yield of N-acetyl-D-glucosamine kinase from rat liver and kidney are described. The separation of this enzyme from hepatic glucokinase depended primarily on their differing behaviour on an affinity column of Sepharose--N-(6-aminohexanoyl)-2-amino-2-deoxy-D-glucopyranose. 2.
Comparative Biochemistry of Molecular Targets for Drug and Pesticides: N-acetyl-D-glucosamine and Acetylcholine Related Enzymes
Yang, Q.Y.
Current Drug Targets (2012)



Global Trade Item Number

SKUGTIN
A2278-1ML04061837765582
A2278-5ML04061833349960
A2278-25ML04061837765599