A2383
Adenosine 5′-triphosphate disodium salt hydrate
Grade I, ≥99%, from microbial
biological source
microbial
Quality Level
type
Grade I
Assay
≥99%
form
powder
solubility
H2O: 50 mg/mL
storage temp.
−20°C
SMILES string
O.[Na+].[Na+].Nc1ncnc2n(cnc12)[C@@H]3O[C@H](COP(O)(=O)OP([O-])(=O)OP(O)([O-])=O)[C@@H](O)[C@H]3O
InChI
1S/C10H16N5O13P3.2Na.H2O/c11-8-5-9(13-2-12-8)15(3-14-5)10-7(17)6(16)4(26-10)1-25-30(21,22)28-31(23,24)27-29(18,19)20;;;/h2-4,6-7,10,16-17H,1H2,(H,21,22)(H,23,24)(H2,11,12,13)(H2,18,19,20);;;1H2/q;2*+1;/p-2/t4-,6-,7-,10-;;;/m1.../s1
InChI key
NTBQNWBHIXNPRU-MSQVLRTGSA-L
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Related Categories
Application
- to prepare adenosine triphosphate (ATP) standard solutions to determine ATP levels in various bacterial cultures
- bone marrow-derived macrophages (BMDM) treatment
- as a preparation free of contaminating guanine nucleotides to study the binding effects of coatomer β subunit (β-COP)
Biochem/physiol Actions
Storage Class Code
11 - Combustible Solids
WGK
WGK 2
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Personal Protective Equipment
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Articles
Separation of AMP (adenosine monophosphate); ATP (adenosine triphosphate)
Learn about the four membrane-bound protein complexes that make up the electron transport chain metabolic pathway supplying energy as ATP for cellular respiration.
Protocols
To measure luciferase activity, an assay that measures light emission with a luminometer is used. Firefly luciferase is used for detection and quantitation of ATP and as a reporter for genetic function.
To measure hexokinase activity, a continuous spectrophotometric rate-determination assay is used at 340 nm. Hexokinase catalyzes the phosphorylation of D-hexose sugars using ATP as the phosphate source.
Enzymatic assay of lipase type XIII from Pseudomonas sp. using a coupled enzyme system of glycerol kinase and glycerophosphate oxidase (EC 3.1.1.3)
Separation of Adenosine 5′-triphosphate disodium salt hydrate, BioXtra, ≥99% (HPLC), from microbial; Adenosine 5′-diphosphate sodium salt, bacterial, ≥95% (HPLC); 2′-Deoxyadenosine 5′-monophosphate, Sigma Grade, 98-100%
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