Quality Level
Assay
≥98% (HPLC)
form
powder
mp
226-232 °C (dec.) (lit.)
antibiotic activity spectrum
viruses
Mode of action
DNA synthesis | interferes
originator
Celgene
storage temp.
−20°C
SMILES string
NC1=NC(=O)N(C=N1)[C@@H]2O[C@H](CO)[C@@H](O)[C@H]2O
InChI
1S/C8H12N4O5/c9-7-10-2-12(8(16)11-7)6-5(15)4(14)3(1-13)17-6/h2-6,13-15H,1H2,(H2,9,11,16)/t3-,4-,5-,6-/m1/s1
InChI key
NMUSYJAQQFHJEW-KVTDHHQDSA-N
Gene Information
human ... DNMT1(1786) , DNMT3A(1788) , DNMT3B(1789)
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General description
Application
- for cell induction
- to study its effects on bovine fetal mesenchymal stem cells (bfMSC)
- to study its effects on human bone marrow stromal cells (hBMSCs)
- to study its effects on and DNA methyltransferase 1 (DNMT1) and Ras protein activator like 1 (RASAL1) expression
- to interfere with DNA methylation and histone acetylation
- to determine its effects on the conversion of control fibroblasts
- for optical coherence tomography (OCT) and fluorescein angiography (FA)
- for the reactivation of Sal-like protein (SALL)3 expression
Biochem/physiol Actions
Features and Benefits
Signal Word
Danger
Hazard Statements
Precautionary Statements
Hazard Classifications
Acute Tox. 4 Oral - Aquatic Chronic 1 - Carc. 1A - Muta. 2 - Repr. 1B - STOT RE 1
Storage Class Code
6.1C - Combustible, acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects
WGK
WGK 3
Personal Protective Equipment
Certificates of Analysis (COA)
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1000309185
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If available for a given product, the recommended re-test date or the expiration date can be found on the Certificate of Analysis.
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What is the solution stability of Product No. A2385? That is, how should I store solutions of 5-Azacytidine?
We generally don't recommend storing solutions, since it has such poor solution stability. In Cancer Res., 61, 6331-6334 (2001), A. Chetcuti et al. describe preparing a 5 micromolar working concentration from a 5 Molar freshly prepared stock solution in DMSO.
What is Product No. A2385 (5-Azacytidine) used for?
This nucleoside analog has been used variously as an antitumor agent and as a mutagenic agent.
I need to perform an identity test on Product No. A2385 (5-Azacytidine). What would you recommend?
This means that each lot is tested according to the list of tests (left hand column), and must meet or exceed the specifications (right hand column). HPLC. The elemental analyses for carbon and nitrogen content are identity tests (albeit not absolutely conclusive identity tests). I would say that the HPLC, using a previous lot of A2385 as a control sample, provides the best evidence for identity and purity.
I just need to perform a purity assay on Product No. A2385 (5-Azacytidine). What would you recommend?
We assay the purity of this product by HPLC. If you would like to obtain a copy of our HPLC parameters/method, please contact your local Sigma-Aldrich Technical Service group.
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