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A2512

Sigma-Aldrich

Albumin from chicken egg white

lyophilized powder, ≥98% (agarose gel electrophoresis)

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Synonym(s):
Albumin from hen egg white, Ovalbumin
CAS Number:
9006-59-1
EC Number:
232-692-7
MDL number:
MFCD00130424
eCl@ss:
42010201
NACRES:
NA.61

biological source

chicken egg white

Quality Level

300

type

Grade VI

Assay

≥98% (agarose gel electrophoresis)

form

lyophilized powder

mol wt

44287 Da by calculation

purified by

chromatography

extent of labeling

4-20 mol mannose per mol ovalbumin protein

technique(s)

bioconjugation: suitable

solubility

H2O: soluble 50 mg/mL, clear to slightly hazy, colorless to faintly yellow

UniProt accession no.

P01012

storage temp.

2-8°C

Gene Information

chicken ... OVAL(396058)

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General description

Chicken egg albumin is the major protein constituent of egg whites. Chicken egg albumin is a phosphorylated-glycoprotein. From the amino acid sequence, the peptide portion of the molecule consists of 385 residues and has a molecular weight of 42.7 kDa.
Ovalbumin makes 75% of the total egg white protein.

Application

Ovalbumin has been used:
  • as a medium for cell incubation in EliCell assay for the detection of released cytokines from eosinophil
  • to induce allergic inflammation and atopic dermatitis in experimental mice
  • as a component in control mixture for validating tandem mass spectral analysis
  • as a model protein antigen to evaluate the in vivo efficacy of a chitosan/interleukin (IL)-12 adjuvant system
A phosphorylated glycoprotein which is useful as a marker (~45 kDa) for protein electrophoresis. Has been used to produce an animal model of asthma.

Biochem/physiol Actions

Albumin from chicken egg white serves as a natural adhesive.
Differential scanning calorimetry indicates that chicken egg albumin denatures at 84 °C. Ovalbumin can be used as a carrier protein to conjugate to synthetic peptides for use as an immunogen. It has the following amino acids: 20 Lys, 10 Tyr, 6 Cys, 14 Asp, and 33 Glu which make it suitable for conjugation.

Physical form

Essentially salt free

Preparation Note

A further purification of A 5503 to reduce mannose content.

Pictograms

Health hazard
GHS08

Signal Word

Danger

Hazard Statements

H334

Hazard Classifications

Resp. Sens. 1A

Storage Class Code

11 - Combustible Solids

WGK

WGK 2

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

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PTP1B deficiency exacerbates inflammation and accelerates leukocyte trafficking in vivo
<BIG>Berdnikovs S, et al.</BIG>
Journal of Immunology, 188, 874-884 (2012)
<BIG>Mielenz JR, et al.</BIG>
Biotechnology for Fuels and Chemicals: The Twenty-Eighth Symposium.c, 928-928 (2019)
<BIG>Unger A, et al.</BIG>
Conservation of Wood Artifacts: A Handbook, 554-554 (2013)
In vivo efficacy of a chitosan/IL-12 adjuvant system for protein-based vaccines
<BIG>Heffernan MJ, et al.</BIG>
Biomaterials, 32, 926-932 (2011)
Experimental protein mixture for validating tandem mass spectral analysis
<BIG>Keller A, et al.</BIG>
Omics : A Journal of Integrative Biology, 6, 207-212 (2002)
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