A2556
Monoclonal Anti-Rabbit IgG (γ-chain specific)−Alkaline Phosphatase antibody produced in mouse
clone RG-96, purified immunoglobulin, buffered aqueous glycerol solution
Synonym(s):
Monoclonal Anti-Rabbit IgG (γ-chain specific)
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About This Item
NACRES:
NA.46
UNSPSC Code:
12352203
Conjugate:
alkaline phosphatase conjugate
Clone:
RG-96, monoclonal
Application:
ELISA (d), IHC (p), WB
Citations:
27
biological source
mouse
conjugate
alkaline phosphatase conjugate
antibody form
purified immunoglobulin
antibody product type
secondary antibodies
clone
RG-96, monoclonal
form
buffered aqueous glycerol solution
species reactivity
rabbit
should not react with
goat, feline, pig, guinea pig, rat, bovine, canine, human, horse, sheep, chicken
technique(s)
direct ELISA: 1:50,000, immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:100, western blot: 1:200,000-1:400,000 using total cell extract of HeLa cells
isotype
IgG1
shipped in
wet ice
storage temp.
2-8°C
target post-translational modification
unmodified
Quality Level
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General description
Monoclonal Anti-Rabbit IgG (mouse IgG1 isotype) is derived from the hybridoma produced by the fusion of mouse myeloma cells and splenocytes from an immunized mouse. Immunoglobulin G (IgG) belongs to the immunoglobulin family and is a widely expressed serum antibody. Immunoglobulin has two heavy chains and two light chains connected by a disulfide bond. It is a glycoprotein and mainly helps in immune defense. IgG is a major class of immunoglobulin. Rabbit IgG is further divided into five classes- IgM, IgG, IgA, IgD and IgE.
The product recognizes an epitope located on the γ (heavy)-chain of rabbit IgG. In immunoblotting, the antibody recognizes both native and denatured forms of rabbit IgG. In ELISA, the antibody is specific for rabbit IgG, and shows no cross-reactivity with rabbit IgA and IgM or human IgG, IgA, and IgM. No cross reaction is observed with IgG from the following species: bovine, cat, chicken, dog, goat, guinea pig, horse, pig, rat, or sheep.
Application
Monoclonal Anti-Rabbit IgG (γ-chain specific)Alkaline Phosphatase antibody produced in mouse has been used in enzyme-linked immunosorbent assay (ELISA) and immunoblotting.
Biochem/physiol Actions
Immunoglobulin G (IgG) participates in hypersensitivity type II and type III. It helps in opsonization, complement fixation and antibody dependent cell mediated cytotoxicity.
Physical form
Solution in 0.05 M Tris buffer, pH 8.0, containing 1 mM MgCl2, 1% bovine serum albumin, 50% glycerol and 15 mM sodium azide.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Storage Class
10 - Combustible liquids
wgk
WGK 2
ppe
Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)
Regulatory Information
含少量动物源组分生物产品
低风险生物材料
常规特殊物品
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Wen-Hao Tang et al.
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Arnold JN, et al.
The Journal of Biological Chemistry, 281(11), 6955-6963 (2006)
Heidi C Schmid et al.
Biological chemistry, 383(11), 1769-1778 (2003-01-18)
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