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Merck
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A2831

Monoclonal Anti-DNP−Alkaline Phosphatase Conjugate antibody produced in mouse

clone SPE-7, purified immunoglobulin, buffered aqueous glycerol solution

Synonym(s):

Anti-Dinitrophenyl, Monoclonal Anti-DNP

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About This Item

NACRES:
NA.46
UNSPSC Code:
12352203

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Product Name

Monoclonal Anti-DNP−Alkaline Phosphatase Conjugate antibody produced in mouse, clone SPE-7, purified immunoglobulin, buffered aqueous glycerol solution

biological source

mouse

conjugate

alkaline phosphatase conjugate

antibody form

purified immunoglobulin

antibody product type

primary antibodies

clone

SPE-7, monoclonal

form

buffered aqueous glycerol solution

technique(s)

direct ELISA: 1:25,000 using DNP-BSA
dot blot: 1:180,000 (chemiluminescence)

isotype

IgE

shipped in

wet ice

storage temp.

2-8°C

target post-translational modification

unmodified

Quality Level

200

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Analysis Note

Monoclonal Anti-DNP is tested to ensure minimal reactivity against bovine serum albumin.

Application

Mouse monoclonal anti-DNP-alkaline phosphatase conjugate antibody can be used for ELISA (1:25,000) and dot blot (1:180,000) assays.
The antibody may be used for experimental induction of controlled allergic reactions or as a source of mouse IgE.
The antibody may be used for experimental induction of controlled allergic reactions or as a source of mouse IgE.To induce systemic anaphylaxis, mice were injected with 3 μg of anti-DNP mouse IgE and then given by IV 500 μg of DNP-BSA twenty hours later. To induce local anaphylaxis, mice were injected intradermally with 10ng of anti-DNP mouse IgE and then challenged with an i.v. injection of 500 μg DNP-BSA.
Used to induce controlled allergic reactions or as a source of mouse IgE.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

General description

Dinitrophenyl (DNP) is a hapten that is used for labeling primary or secondary probes in immunological analyses. Thus, anti-DNP antibody conjugated to a detectable substrate is a useful tool for the study of target proteins. Mouse monoclonal anti-DNP-alkaline phosphatase conjugate antibody is specific for DNP.

Immunogen

DNP-KLH conjugate

Physical form

Solution in 0.05 M Tris buffer, pH 8.0, containing 1 mM MgCl2, 1% BSA, and 50% glycerol and 15 mM Sodium azide

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Storage Class

10 - Combustible liquids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)

Regulatory Information

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Certificates of Analysis (COA)

Lot/Batch Number
0000509366
0000509366
0000298382
0000156901
0000142967

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Urszula Krasuska et al.
Frontiers in plant science, 7, 840-840 (2016-07-06)
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ACS omega, 8(32), 29812-29835 (2023-08-21)
Pesticides are widely used, resulting in continuing human exposure with potential health impacts. Some exposures related to agricultural works have been associated with neurological disorders. Since the 2000s, the hypothesis of the role of pesticides in the occurrence of central
B Jasani et al.
Journal of immunological methods, 150(1-2), 193-198 (1992-06-24)
Over the past 10 years an immunoperoxidase method using dinitrophenyl (DNP) hapten-labelled primary or secondary probes has been devised. Its widely successful application in research and diagnostic work has depended upon the development of certain key reagents. These include a
Katarzyna Ciacka et al.
Antioxidants (Basel, Switzerland), 11(1) (2022-01-22)
Seed ageing is associated with a high concentration of reactive oxygen species (ROS). Apple (Malus domestica Borkh.) seeds belong to the orthodox type. Due to a deep dormancy, they may be stored in dry condition at 5 °C for a
Sandro Capellmann et al.
Frontiers in immunology, 14, 1154416-1154416 (2023-04-18)
Mast cells (MCs) are immune cells of the myeloid lineage distributed in tissues throughout the body. Phenotypically, they are a heterogeneous group characterized by different protease repertoires stored in secretory granules and differential presence of receptors. To adequately address aspects

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Data Sheet - A2831

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