Anti-Human Lambda Light Chains (Bound and Free)−Alkaline Phosphatase antibody produced in goat

Immunoglobulins are glycoprotein antibodies that contain two equivalent light chains and a pair of identical heavy chains. Immunoglobulin light chains are the primary protein components of the amyloid fibrillar substance that is present in patients having myeloma-associated amyloidosis . Furthermore, urinary free immunoglobulin light chains can be used for the immunodiagnosis of Bence Jones proteinuria . Anti-Human λ Light Chains (Bound and Free)-Alkaline Phosphatase antibodies are specific for human λ light chains when tested against bound κ and λ proteins (human IgA, IgG, IgM) and free Bence Jones κ and λ myeloma proteins.

Mammalian immunoglobins contain either lambda or kappa light chains.
Alkaline Phosphatase is an enzyme that catalyzes the conversion of chromogenic substrates such as p-nitrophenylphosphate (PNPP); chemiluminescent substrates such as CDP-Star^® and fluorogenic substrates such as 4-methylumbelliferyl phosphate (4-MUP) into detectable chromophores, light-emitters or fluorescers, respectively.

λ light chains isolated from Bence Jones urines

Goat polyclonal anti-Human Lambda Light Chains (Bound and Free)-Alkaline Phosphatase antibody may be used to detect human lamba light chain containing immunoglobulins by chromogenic, fluorogenic, and chemiluminescent techniques. Whole cell bacterial ELISAs were performed to detect the light and heavy fragments of monoclonal antibodies against the meningococcal porA protein. The alkaline phosphatase conjugated goat anti-human λ Light Chains IgG was used as the secondary. The reaction was developed using p-nitrophenyl phosphate substrate (Sigma).

Solution in 0.05 M Tris, pH 8.0, containing 1% bovine serum albumin, 1 mM MgCl₂, 50% glycerol and 15 mM sodium azide

CDP-Star is a registered trademark of Tropix, Inc.

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