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Merck
CN

A3157

Alcian Blue 8GX

Dye content, ≥50%, certified by the Biological Stain Commission, powder

Synonym(s):

Alcian Blue, Ingrain Blue 1

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About This Item

Empirical Formula (Hill Notation):
C56H68Cl4CuN16S4
CAS Number:
33864-99-2
Molecular Weight:
1298.86
NACRES:
NA.47
PubChem Substance ID:
24890721
UNSPSC Code:
12171500
EC Number:
251-705-7
MDL number:
MFCD00010720
Colour Index Number:
74240

Key Documents

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Product Name

Alcian Blue 8GX, certified by the BSC

InChI key

CKLBXIYTBHXJEH-UHFFFAOYSA-J

InChI

1S/C56H68N16S4.4ClH.Cu/c1-65(2)53(66(3)4)73-29-33-17-21-37-41(25-33)49-57-45(37)62-50-43-27-35(31-75-55(69(9)10)70(11)12)19-23-39(43)47(59-50)64-52-44-28-36(32-76-56(71(13)14)72(15)16)20-24-40(44)48(60-52)63-51-42-26-34(18-22-38(42)46(58-51)61-49)30-74-54(67(5)6)68(7)8;;;;;/h17-28H,29-32H2,1-16H3;4*1H;/q+2;;;;;+2/p-4

SMILES string

[Cl-].[Cl-].[Cl-].[Cl-].CN(C)C(\SCc1ccc2c3nc(nc4n5[Cu]n6c(nc7nc(nc5c8cc(CS\C(N(C)C)=[N+](\C)C)ccc48)c9ccc(CS\C(N(C)C)=[N+](\C)C)cc79)c%10ccc(CS\C(N(C)C)=[N+](\C)C)cc%10c6n3)c2c1)=[N+](/C)C

agency

certified by the BSC

form

powder

composition

Dye content, ≥50%

technique(s)

microbe id | staining: suitable

color

dark blue

solubility

water: 1 mg/mL, blue

εmax

195-400 at 617-630 nm

application(s)

diagnostic assay manufacturing
hematology
histology

storage temp.

room temp

Quality Level

200

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Application

Alcian Blue 8GX has been used for the staining of sulfated proteoglycans in murine MPC (mesenchymal progenitor cell) nodule. It has been used for the staining of sulfated proteoglycans in bovine articular chondrocytes.
Certified for use in staining in Mowry′s pH 2.5 method, in Scott′s pH 5.7 method, and in Kreyberg′s method for keratin and mucin.

Biochem/physiol Actions

Alcian Blue 8GX is a cationic dye. It forms insoluble complexes with acidic glycosaminoglycans, thereby helping in the quantitative determination of glycosaminoglycans. It is also used for the staining of bone and cartilage.

Storage Class

11 - Combustible Solids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)

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Certificates of Analysis (COA)

Lot/Batch Number
SHBS8900
SHBS3495
SHBR9076
SHBQ4515
SHBP5916

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Chitosan/polyester-based scaffolds for cartilage tissue engineering: assessment of extracellular matrix formation.
Alves da Silva ML, et al.
Acta Biomaterialia, 6, 1149-1157 (2010)
Isolation of mouse marrow mesenchymal progenitors by a novel and reliable method.
Sun S, et al.
Stem Cells, 21, 527-535 (2003)
The quantitative determination of glycosaminoglycans in urine with Alcian Blue 8GX.
Whiteman P
The Biochemical Journal, 131, 351-357 (1973)
Virginie Tissières et al.
Cell reports, 31(1), 107490-107490 (2020-04-09)
Digit loss/reductions are evolutionary adaptations in cursorial mammals such as pigs. To gain mechanistic insight into these processes, we performed a comparative molecular analysis of limb development in mouse and pig embryos, which revealed a loss of anterior-posterior polarity during
Lara Vlietstra et al.
PloS one, 14(6), e0217462-e0217462 (2019-06-07)
To determine if there is an association between sarcopenia, physical function and self-reported fatigue in osteoarthritis (OA) and rheumatoid arthritis (RA). A cross-sectional analysis of measurements from a cohort of 157 participants with OA or RA was performed. The relationship
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