A3401
Ala-Ala-Phe-7-amido-4-methylcoumarin
protease substrate
Synonym(s):
H-Ala-Ala-Phe-AMC
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About This Item
Empirical Formula (Hill Notation):
C25H28N4O5
CAS Number:
Molecular Weight:
464.51
NACRES:
NA.32
PubChem Substance ID:
UNSPSC Code:
12352204
MDL number:
Quality Segment
assay
≥98% (TLC)
form
powder
solubility
ethanol: 20 mg/mL, clear, colorless to light yellow
storage temp.
2-8°C
SMILES string
CC(N)C(=O)NC(C)C(=O)NC(Cc1ccccc1)C(=O)Nc2ccc3C(C)=CC(=O)Oc3c2
InChI
1S/C25H28N4O5/c1-14-11-22(30)34-21-13-18(9-10-19(14)21)28-25(33)20(12-17-7-5-4-6-8-17)29-24(32)16(3)27-23(31)15(2)26/h4-11,13,15-16,20H,12,26H2,1-3H3,(H,27,31)(H,28,33)(H,29,32)
InChI key
FVRLYIFIDKXFHU-UHFFFAOYSA-N
General description
Ala-Ala-Phe-7-amido-4-methylcoumarin is a fluorogenic substrate for the proteolytic activity. It is a positively charged substrate molecule.
Substrate for chymotrypsin and tripeptidyl peptidaseII.
Application
Ala-Ala-Phe-7-amido-4-methylcoumarin has been used:
- in the preparation of the reaction mixture for enzymatic assays
- to initiate the enzyme reaction in tripeptidyl peptidase-1 (TPP1) enzyme activity assay
- to initiate the assay reaction in lysosomal hydrolases activity assay
Storage Class
11 - Combustible Solids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, type N95 (US)
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