A3415
Anti-Sheep IgG (whole molecule)–Peroxidase antibody produced in donkey
affinity isolated antibody, buffered aqueous solution
Synonym(s):
Anti Sheep Antibody, Anti Sheep Antibody - Anti-Sheep IgG (whole molecule)–Peroxidase antibody produced in donkey, Anti Sheep Hrp
biological source
donkey
Quality Level
conjugate
peroxidase conjugate
antibody form
affinity isolated antibody
antibody product type
secondary antibodies
clone
polyclonal
form
buffered aqueous solution
species reactivity
sheep
technique(s)
direct ELISA: 1:10,000
shipped in
dry ice
storage temp.
−20°C
target post-translational modification
unmodified
Related Categories
General description
IgGs are glycoprotein antibodies that modulate several immune responses. Sheep IgGs against target proteins are often used as primary antibodies in various research applications. Thus, secondary anti-sheep IgGs conjugated to a detectable substrate are useful tools for the analysis of target proteins. Anti-Sheep IgG (whole molecule)-Peroxidase antibody reacts with all sheep immunoglobulins.
Immunogen
Purified sheep IgG
Application
Anti-Sheep IgG (whole molecule)-Peroxidase antibody is suitable for use in immunoblot.
Applications in which this antibody has been used successfully, and the associated peer-reviewed papers, are given below.
Western Blotting (1 paper)
Western Blotting (1 paper)
rElisas and CHEkiT Elisas were performed on serum from Sarda milking ewes using HRP-conjugated donkey anti-sheep IgG at1:1000. The antibody was incubated for 30 minutes at 37 degrees.
Physical form
Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 1% bovine serum albumin with preservative
Preparation Note
Prepared using the periodate method described by Wilson, M.B., and Nakane, P.K., in Immunofluorescence and Related Staining Techniques, Elsevier/North Holland Biomedical Press, Amsterdam, p215 (1978).
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Signal Word
Warning
Hazard Statements
Precautionary Statements
Hazard Classifications
Aquatic Chronic 2 - Eye Irrit. 2 - Skin Irrit. 2 - Skin Sens. 1
Storage Class Code
12 - Non Combustible Liquids
WGK
WGK 3
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Regulatory Information
常规特殊物品
含少量动物源组分生物产品
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Marcus G Davey et al.
PloS one, 12(1), e0171132-e0171132 (2017-02-01)
A major limitation to adeno-associated virus (AAV) gene therapy is the generation of host immune responses to viral vector antigens and the transgene product. The ability to induce immune tolerance to foreign protein has the potential to overcome this host
Increased contractility of diabetic rabbit corpora smooth muscle in response to endothelin is mediated via Rho-kinase β
Chang, S., et al.
The Journal of Urology, 15, 53-62 (2003)
Max Werth et al.
Hepatology (Baltimore, Md.), 44(4), 967-975 (2006-09-29)
In mammalian liver, high glutamine synthetase (GS) expression is restricted to hepatocytes surrounding the terminal venules. The most important enhancer of the GS gene is located approximately 2520 base pairs (bp) upstream from the transcriptional start point. The nature of
ACE inhibition increases expression of the ETB receptor in kidneys of mice with unilateral obstruction.
Moridaira, K., et al.
American Journal of Physiology: Renal Physiology, 284(1), F209-F217 (2003)
María J Torres et al.
Frontiers in microbiology, 8, 1621-1621 (2017-09-16)
The powerful greenhouse gas, nitrous oxide (N
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