A3422

Achromopeptidase from bacteria

Name: Achromopeptidase from bacteria
Brand: SIGMA

partially purified powder, ≥20,000 units/mg solid

Synonym(s):

ACP, Achromopeptidase

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About This Item

EC Number:

3.4.21.50 (BRENDA, IUBMB)

MDL number:

MFCD00130333

UNSPSC Code:

12352204

NACRES:

NA.54

Key Documents

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form

partially purified powder

Quality Level

200

specific activity

≥20,000 units/mg solid

mol wt

27 kDa

foreign activity

Collagenase, present

storage temp.

−20°C

Application

Achromopeptidase from bacteria may be used:

in the homogenization step for genomic DNA extraction from microbial biofilm
in the lysis of gram-positive strains(
for DNA extraction from Nocardia strains

Achromopeptidase is useful for lysis of Gram-positive bacteria that are resistant to lysozyme. Achromopeptidase, from Sigma, has been used for the isolation and molecular characterization of skin and soft-tissue bacterial infections. Product A3422 is from bacteria and is a partially purified powder containing lactose.

Biochem/physiol Actions

Achromopeptidase is a bacteriolytic enzyme and belongs to serine protease family. It is effective for the lysis of gram positive bacteria compared to lysozyme. Achromopeptidase activity is inhibited in the presence of heavy metals. Achromopeptidase based inhibition assay may be useful for screening the presence of heavy metals.

Achromopeptidase is a lysyl endopeptidase that has a molecular weight of approximately 27 kDa. It works best between pH 8.5 -9. Approximately 500-1,500 μn/ml achromopetidase can be used to lyse cells at a density of OD₆₀₀= 0.6 over 2 hours at 37 °C.

Physical form

Partially purified powder.

Other Notes

One unit will lyse 0.4 μg of Micrococcus lysodeikticus per minute by turbidimetric detection at 600 nm when suspended in buffer at pH 8.0 at 37 °C.

Pictograms

GHS08

Signal Word

Danger

Hazard Statements

H334

Precautionary Statements

P261 - P342 + P311

Hazard Classifications

Resp. Sens. 1

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Regulatory Information

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Metagenomic analysis of an ecological wastewater treatment plant?s microbial communities and their potential to metabolize pharmaceuticals

Balcom IN, et al.

F1000Research, 5, 844-846 (2016)

Microbial ecosystem analysis in root canal infections refractory to endodontic treatment

Henriques LC, et al.

Journal of Endodontics, 42(8), 1239-1245 (2016)

High Intraspecific Genetic Diversity of Nocardia brasiliensis, a Pathogen Responsible for Cutaneous Nocardiosis Found in France: Phylogenetic Relationships by Using sod and hsp65 Genes

Kosova-Maali D, et al.

BioMed Research International, 2018 (2018)

Achromopeptidase for lysis of anaerobic gram-positive cocci.

Ezaki T and Suzuki S

Journal of Clinical Microbiology, 16(5), 844-846 (1982)

Near real-time inhibitive assay for heavy metals using achromopeptidase

Shukor MY, et al.

Indian Journal of Biotechnology, 13, 398-403 (2014)

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Protocols

Enzymatic Assay of Achromopeptidase

To measure achromopeptidase activity, this procedure uses bacterial cells and a turbidimetric rate assay. Turbidity is measured at 600 nm using a spectrophotometer.

Achromopeptidase from bacteria

partially purified powder, ≥20,000 units/mg solid

Select a Size

About This Item

Key Documents

Properties

form

Quality Level

specific activity

mol wt

foreign activity

storage temp.

Description

Application

Biochem/physiol Actions

Physical form

Other Notes

Safety Information

Pictograms

Signal Word

Hazard Statements

Precautionary Statements

Hazard Classifications

Storage Class Code

WGK

Flash Point(F)

Flash Point(C)

Personal Protective Equipment

Regulatory Information

Documentation

Certificates of Analysis (COA)

Already Own This Product?

Peer Reviewed Papers

Protocols and Articles

Protocols

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