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A3678

Ammonium persulfate

Name: Ammonium persulfate
Brand: SIGMA

Molecular Biology, suitable for electrophoresis, ≥98%

Synonym(s):

AP, APS, Ammonium peroxodisulfate, Ammonium peroxydisulfate, PER

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About This Item

Linear Formula:

(NH₄)₂S₂O₈

CAS Number:

7727-54-0

Molecular Weight:

228.20

PubChem Substance ID:

24890800

eCl@ss:

38030421

UNSPSC Code:

41105319

NACRES:

NB.22

EC Number:

231-786-5

MDL number:

MFCD00003390

Assay:

≥98%

Form:

powder

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grade

Molecular Biology

Quality Level

200

vapor density

7.9 (vs air)

assay

≥98%

form

powder

reaction suitability

reagent type: oxidant

technique(s)

electrophoresis: suitable

anion traces

chloride (Cl^-): <10 ppm

cation traces

Fe: <10 ppm, heavy metals (as Pb): <50 ppm

foreign activity

DNAse, none detected, Protease, none detected, RNAse, none detected

SMILES string

N.N.OS(=O)(=O)OOS(O)(=O)=O

InChI

1S/2H3N.H2O8S2/c;;1-9(2,3)7-8-10(4,5)6/h2*1H3;(H,1,2,3)(H,4,5,6)

InChI key

ROOXNKNUYICQNP-UHFFFAOYSA-N

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Chemical Synthesis

Oxidation Reagents

General description

Ammonium persulfate (APS) is a widely used reagent in biochemistry and molecular biology for the preparation of polyacrylamide gels. APS forms oxygen free radicals in aqueous solution by a base-catalyzed mechanism. The bases, most commonly used as catalysts, are tertiary amines such as TEMED (N,N,N′,N′-tetramethylethylenediamine) or DMAPN (3-dimethylaminopropionitrile). The free radicals will cause the polymerization of acrylamide and bis-acrylamide to form a gel matrix, which can be used for separating macromolecules by size. APS has also been utilized to study protein-protein interactions via photoinitiated crosslinking chemistry.

Application

Ammonium persulfate has been used for the preparation of polyacrylamide gels and acrylamide hydrogels.

Catalyst for acrylamide gel polymerization.

Analysis Note

Tested for use in acrylamide polymerization.

pictograms

GHS03,GHS08,GHS07

signalword

Danger

hcodes

H272,H302,H315,H317,H319,H334,H335

pcodes

P210 - P220 - P280 - P301 + P312 - P302 + P352 - P305 + P351 + P338

Hazard Classifications

Acute Tox. 4 Oral - Eye Irrit. 2 - Ox. Sol. 3 - Resp. Sens. 1 - Skin Irrit. 2 - Skin Sens. 1 - STOT SE 3

target_organs

Respiratory system

Storage Class

5.1B - Oxidizing hazardous materials

wgk

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Faceshields, Gloves, type P3 (EN 143) respirator cartridges

Regulatory Information

危险化学品

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Chemistry for the analysis of protein-protein interactions: rapid and efficient cross-linking triggered by long wavelength light.

Fancy DA and Kodadek T

Proceedings of the National Academy of Sciences of the USA, 96, 6020-6020 (1999)

Relationships among cell morphology, intrinsic cell stiffness and cell-substrate interactions.

Chiang MY et al.

Biomaterials, 34, 9754-9754 (2013)

Maurer HR

Disc Electrophoresis and Related Techniques of Polyacrylamide Gel Electrophoresis (1978)

Development of digestive enzyme activity in larvae of spotted sand bass Paralabrax maculatofasciatus II: Electrophoretic analysis.

Alvarez-Gonzalez CA, et al.

Fish Physiology and Biochemistry, 36, 29-29 (2010)

Composite three-dimensional woven scaffolds with interpenetrating network hydrogels to create functional synthetic articular cartilage.

Liao IC et al.

Advances in Functional Materials, 23, 5833-5833 (2013)

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Protocols

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Simplify your SDS-PAGE gel preparation with our pre-mixed solutions and protocols to achieve precise protein separation.

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Global Trade Item Number

SKU	GTIN
A3678-25G	04061835515707
A3678-100G	04061835521531

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