A3861
Anti-Palladin (C-terminal) antibody produced in rabbit
IgG fraction of antiserum, buffered aqueous solution
Synonym(s):
Anti-PALLD
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About This Item
NACRES:
NA.41
UNSPSC Code:
12352203
Conjugate:
unconjugated
Clone:
polyclonal
Application:
immunoprecipitation (IP)
indirect immunofluorescence
western blot
indirect immunofluorescence
western blot
Species reactivity:
human
Citations:
6
Technique(s):
immunoprecipitation (IP): 10-20 μL using HeLa cell lysates
indirect immunofluorescence: 1:250-1:500 using human HeLa cells
western blot: 1:250-1:500 using whole extracts of human HeLa cells
indirect immunofluorescence: 1:250-1:500 using human HeLa cells
western blot: 1:250-1:500 using whole extracts of human HeLa cells
Uniprot accession no.:
Product Name
Anti-Palladin (C-terminal) antibody produced in rabbit, IgG fraction of antiserum, buffered aqueous solution
biological source
rabbit
conjugate
unconjugated
antibody form
IgG fraction of antiserum
antibody product type
primary antibodies
clone
polyclonal
form
buffered aqueous solution
mol wt
antigen ~140 kDa (another band at 90 kDa)
species reactivity
human
packaging
antibody small pack of 25 μL
technique(s)
immunoprecipitation (IP): 10-20 μL using HeLa cell lysates
indirect immunofluorescence: 1:250-1:500 using human HeLa cells
western blot: 1:250-1:500 using whole extracts of human HeLa cells
UniProt accession no.
shipped in
dry ice
storage temp.
−20°C
target post-translational modification
unmodified
Quality Level
Gene Information
human ... PALLD(23022)
mouse ... Palld(72333)
Application
Anti-Palladin (C-terminal) antibody produced in rabbit has been used in:
- immunoblotting
- immunoprecipitation
- immunofluorescence
Rabbit anti-Palladin (C-terminal) antibody can be used for immunoprecipitation (10-20μL), indirect immunofluorescence (1:250-1:500) and western blot (1:250 -1:500) using HeLa cells.
Biochem/physiol Actions
Palladin is a component of actin-containing microfilaments that control cell shape, adhesion, and contraction. Its expression is required for normal mammalian embryogenesis since inactivation of paladin leads to embryonic lethality. Palladin interacts with the actin-associated proteins -actinin, VASP, ezrin, and profilin, suggesting that it is required for normal actin cytoskeleton organization. It serves as a transcriptional regulator, which controls the expression of smooth muscle cell genes.
Palladin is a microfilament-associated phosphoprotein that is known to colocalize with alpha-actinin at cell-cell junctions, stress fibres, embryonic Z-lines, and focal adhesions. Palladin abnormalities have been linked to familial pancreatic cancer. Rabbit anti-Palladin (C-terminal) antibody binds to human palladin. Detection of the palladin band by immunoblotting is specifically inhibited by the immunizing peptide. A nonspecific band of approx. 55kDa may be detected in some cell extract preparations.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
General description
Anti-Palladin (C-terminal) is produced in rabbit using as the immunogen a synthetic peptide corresponding to a sequence at the C-terminal of human palladin, conjugated to KLH. Palladin, an actin associated Ig-domain protein is mapped to human chromosome 4q32. This phosphoprotein is widely expressed in vertebrate cells and tissues. Palladin is expressed as three major isoforms of 90, 140 and 200 kDa.
Physical form
Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.
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Storage Class
12 - Non Combustible Liquids
wgk
WGK 2
Regulatory Information
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Meta-analysis of sex differences in gene expression in schizophrenia
BMC Systems Biology, 10(1), S9-S9 (2016)
Palladin mediates stiffness-induced fibroblast activation in the tumor microenvironment
McLane JS and Ligon LA
Biophysical Journal, 109(2), 249-264 (2015)
The actin associated protein palladin is important for the early smooth muscle cell differentiation
Jin L, et al.
PLoS ONE, 5(9), e12823-e12823 (2010)
Kay L Pogue-Geile et al.
PLoS medicine, 3(12), e516-e516 (2006-12-30)
Pancreatic cancer is a deadly disease. Discovery of the mutated genes that cause the inherited form(s) of the disease may shed light on the mechanism(s) of oncogenesis. Previously we isolated a susceptibility locus for familial pancreatic cancer to chromosome location
Characterization of human palladin, a microfilament-associated protein
Mykka?nen OM, et al.
Molecular Biology of the Cell, 12(10), 3060-3073 (2001)
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