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Merck
CN

A4043

Azure B

Dye content, ≥80%, certified by the Biological Stain Commission, powder

Synonym(s):

N,N,N′-Trimethylthionin, Azure I, Methyleneazure

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About This Item

Empirical Formula (Hill Notation):
C15H16ClN3S
CAS Number:
531-55-5
Molecular Weight:
305.83
UNSPSC Code:
12171500
NACRES:
NA.47
PubChem Substance ID:
24890740
EC Number:
208-511-2
MDL number:
MFCD00011935
Colour Index Number:
52010
Beilstein/REAXYS Number:
3922630

Key Documents

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Product Name

Azure B, certified by the BSC

InChI key

DNDJEIWCTMMZBX-UHFFFAOYSA-N

InChI

1S/C15H15N3S.ClH/c1-16-10-4-6-12-14(8-10)19-15-9-11(18(2)3)5-7-13(15)17-12;/h4-9H,1-3H3;1H

SMILES string

C[N+](C)=C(C=C1)C=C(C1=N2)SC3=C2C=CC(NC)=C3.[Cl-]

agency

certified by the BSC

form

powder

composition

Dye content, ≥80%

technique(s)

microbe id | staining: suitable

color

dark green

mp

205-210 °C (dec.) (lit.)

solubility

H2O: 1 mg/mL

fluorescence

λex 648 in ethanol

application(s)

diagnostic assay manufacturing
hematology
histology

storage temp.

room temp

Quality Level

200

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Related Categories

Application

Azure B has been used for the staining of chromosomes.
Certified for use in Lillie′s modified Nocht′s method for paraffin sections and the NCCLS method for blood smears.

Biochem/physiol Actions

Azure B is a cationic dye. It is part of the Romanowsky stain, which is used for the staining of blood films. It is also used for the localization of nucleic acids.

pictograms

Exclamation mark
GHS07

signalword

Warning

hcodes

H302

Hazard Classifications

Acute Tox. 4 Oral

Storage Class

11 - Combustible Solids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)

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Certificates of Analysis (COA)

Lot/Batch Number
SHBS7221
SHBS4089
SHBP1854
SHBP9824
SHBP3929

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Nuclear DNA content, base composition, and cytogenetic characterization of Christia obcordata.
Sakhanokho HG and Islam-Faridi N
J. Am. Soc. Hort. Sci., 138, 205-209 (2013)
Bain BJ, et al.
Dacie and Lewis Practical Haematology (2016)
E Rosales et al.
Environmental science and pollution research international, 19(5), 1738-1746 (2011-12-14)
The aim of this work was to improve the ability of electro-Fenton technique for the remediation of wastewater contaminated with synthetic dyes using a model azo dye such as Azure B. Batch experiments were conducted to study the effects of
Luaine Bandounas et al.
New biotechnology, 30(2), 196-204 (2012-05-09)
In this study we have investigated the molecular background of the previously reported dye decolourization potential of Bacillus sp. LD003. Strain LD003 was previously isolated on Kraft lignin and was able to decolourize various lignin model dyes. Specifically Azure B
Cell and Tissue Culture in Forestry (2012)
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