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A4043

Azure B

Name: Azure B
Brand: SIGMA

Dye content, ≥80%, certified by the Biological Stain Commission, powder

Synonym(s):

N,N,N′-Trimethylthionin, Azure I, Methyleneazure

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About This Item

Empirical Formula (Hill Notation):

C₁₅H₁₆ClN₃S

CAS Number:

531-55-5

Molecular Weight:

305.83

UNSPSC Code:

12171500

NACRES:

NA.47

PubChem Substance ID:

24890740

EC Number:

208-511-2

MDL number:

MFCD00011935

Colour Index Number:

52010

Beilstein/REAXYS Number:

3922630

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Product Name

Azure B, certified by the BSC

agency

certified by the BSC

Quality Level

200

form

powder

composition

Dye content, ≥80%

technique(s)

microbe id | staining: suitable

color

dark green

mp

205-210 °C (dec.) (lit.)

solubility

H₂O: 1 mg/mL

fluorescence

λ_ex 648 in ethanol

application(s)

diagnostic assay manufacturing
hematology
histology

storage temp.

room temp

SMILES string

C[N+](C)=C(C=C1)C=C(C1=N2)SC3=C2C=CC(NC)=C3.[Cl-]

InChI

1S/C15H15N3S.ClH/c1-16-10-4-6-12-14(8-10)19-15-9-11(18(2)3)5-7-13(15)17-12;/h4-9H,1-3H3;1H

InChI key

DNDJEIWCTMMZBX-UHFFFAOYSA-N

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Related Categories

Clinical & Diagnostics

Dry Dyes & Certified Stains

Hematology Stains

Tissue Diagnostics

Application

Azure B has been used for the staining of chromosomes.

Certified for use in Lillie′s modified Nocht′s method for paraffin sections and the NCCLS method for blood smears.

Biochem/physiol Actions

Azure B is a cationic dye. It is part of the Romanowsky stain, which is used for the staining of blood films. It is also used for the localization of nucleic acids.

pictograms

GHS07

signalword

Warning

hcodes

H302

pcodes

P264 - P270 - P301 + P312 - P501

Hazard Classifications

Acute Tox. 4 Oral

Storage Class

11 - Combustible Solids

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)

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Nuclear DNA content, base composition, and cytogenetic characterization of Christia obcordata.

Sakhanokho HG and Islam-Faridi N

J. Am. Soc. Hort. Sci., 138, 205-209 (2013)

Bain BJ, et al.

Dacie and Lewis Practical Haematology (2016)

A new assay for lignin-type peroxidases employing the dye azure B.

F S Archibald

Applied and environmental microbiology, 58(9), 3110-3116 (1992-09-01)

The discovery in 1983 of fungal "ligninases" capable of catalyzing the peroxidation of nonphenolic aromatic lignin components has been seen as a major advance in understanding how certain basidiomycete fungi can completely degrade lignin. The ability of these lignin-type peroxidases

A comparison of melanin bleaching and azure blue counterstaining in the immunohistochemical diagnosis of malignant melanoma.

C J Kligora et al.

Modern pathology : an official journal of the United States and Canadian Academy of Pathology, Inc, 12(12), 1143-1147 (2000-01-05)

Distinguishing heavily pigmented melanocytes from melanophages on routine hematoxylin and eosin slides can be difficult. Melanin bleaching with potassium permanganate solution is a traditional means of removing melanin from tissues and can be used before immunohistochemical staining to remove any

Azure B, a metabolite of methylene blue, is a high-potency, reversible inhibitor of monoamine oxidase.

Anél Petzer et al.

Toxicology and applied pharmacology, 258(3), 403-409 (2011-12-27)

Methylene blue (MB) has been shown to act at multiple cellular and molecular targets and as a result possesses diverse medical applications. Among these is a high potency reversible inhibition of monoamine oxidase A (MAO-A) that may, at least in

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Global Trade Item Number

SKU	GTIN
A4043-25G	04061835570973
A4043-5G	04061835570980

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