biological source
Aequorea sp.
type
Type III
form
solid
usage
mg solid sufficient for ~200 calcium assays
composition
Protein, ~0.5% Bradford
application(s)
detection
storage temp.
−20°C
General description
Apoaequorin has a mol. wt. approx. 22 kDa, and contains a molecule of coelenterazine hydroperoxide as the chromophoric prosthetic group. Upon binding Ca2+, aequorin decomposes to apoaequorin, coelenteramide, and CO2, and emits light.
Package sizes are based on total solids content
Biochem/physiol Actions
A bioluminescent protein; can be used to measure physiological levels of calcium in serum and subcellular organelles down to 10 μM.
Physical form
Contains buffer salts consisting mainly of sodium EDTA, pH 7.5
Storage Class
11 - Combustible Solids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, type N95 (US)
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