A4140
Aequorin from jellyfish (Aequorea sp.)
Type III, solid
biological source
Aequorea sp.
type
Type III
form
solid
usage
mg solid sufficient for ~200 calcium assays
composition
Protein, ~0.5% Bradford
application(s)
detection
storage temp.
−20°C
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General description
Apoaequorin has a mol. wt. approx. 22 kDa, and contains a molecule of coelenterazine hydroperoxide as the chromophoric prosthetic group. Upon binding Ca2+, aequorin decomposes to apoaequorin, coelenteramide, and CO2, and emits light.
Package sizes are based on total solids content
Biochem/physiol Actions
A bioluminescent protein; can be used to measure physiological levels of calcium in serum and subcellular organelles down to 10 μM.
Physical form
Contains buffer salts consisting mainly of sodium EDTA, pH 7.5
Storage Class
11 - Combustible Solids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, type N95 (US)
Regulatory Information
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ALSUntangled no. 18: apoaequorin (Prevagen).
Amyotrophic lateral sclerosis & frontotemporal degeneration, 14(1), 78-79 (2012-10-04)
Elzbieta Hejchman et al.
The Journal of pharmacology and experimental therapeutics, 343(2), 380-388 (2012-08-16)
We found that amiodarone has potent antifungal activity against a broad range of fungi, potentially defining a new class of antimycotics. Investigations into its molecular mechanisms showed amiodarone mobilized intracellular Ca2+, which is thought to be an important antifungal characteristic
Norbert Mehlmer et al.
Journal of experimental botany, 63(4), 1751-1761 (2012-01-04)
Calcium has long been acknowledged as one of the most important signalling components in plants. Many abiotic and biotic stimuli are transduced into a cellular response by temporal and spatial changes in cellular calcium concentration and the calcium-sensitive protein aequorin
Satoshi Inouye et al.
Protein expression and purification, 83(2), 205-210 (2012-04-28)
Highly purified histidine-tagged aequorin with a reactive cysteine residue (His-Cys4-aequorin) was obtained from the periplasmic space of Escherichia coli cells by nickel-chelate affinity chromatography and hydrophobic chromatography. The procedure yielded 40.3mg of His-Cys4-aequorin from 2L of cultured cells with over
Fen-Fen Lin et al.
The Journal of pharmacology and experimental therapeutics, 345(2), 225-238 (2013-03-12)
Calcium entry into T cells following antigen stimulation is crucial for nuclear factor of activated T cells (NFAT)-mediated T cell activation. The movement of calcium is mediated by calcium release-activated calcium (CRAC) channels. There are two key components of this
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