A4227
Anti-ASAP1/Centaurin β4 (ED-20) antibody produced in rabbit
IgG fraction of antiserum, buffered aqueous solution
Synonym(s):
Anti-Arf GAP containing SH3, ANK repeat, and PH domains/Centaurin β4 (ED-20), DEF1
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About This Item
Conjugate:
unconjugated
Clone:
polyclonal
Application:
ARR, IF, WB
Citations:
-
biological source
rabbit
conjugate
unconjugated
antibody form
IgG fraction of antiserum
antibody product type
primary antibodies
clone
polyclonal
form
buffered aqueous solution
mol wt
antigen 130 kDa (doublet band)
species reactivity
rat, mouse, human
technique(s)
immunofluorescence: 1:1,000 using mouse NIH3T3 fibroblast cells, microarray: suitable, western blot: 1:2,500 using rat brain and mouse brain extracts
UniProt accession no.
shipped in
dry ice
storage temp.
−20°C
target post-translational modification
unmodified
Gene Information
human ... ASAP1(50807)
mouse ... Asap1(13196)
rat ... Asap1(314961)
General description
ASAP1 is a phospholipid-dependent ARF GTPase-activating protein that activates ARF for membrane trafficking regulation.
Rabbit anti-ASAP1/Centaurin β4 (ED-20) antibody recognizes human ASAP1 by immunoblotting (130kDa, doublet band). Staining of ASAP1 in immunoblotting is specifically inhibited with the ASAP1 immunizing peptide.
Immunogen
synthetic peptide located near the C-terminus of mouse ASAP1 (amino acids 1128-1147) conjugated to KLH.
Application
Rabbit anti-ASAP1/Centaurin β4 (ED-20) antibody can be used for immunofluorescence (1:1,000 using mouse NIH3T3 fibroblast cells) and western blot (1:2,000 using rat brain and mouse brain extracts) assays. It is also suitable for use in microarray applications.
Physical form
Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Storage Class
10 - Combustible liquids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
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