A4335
Monoclonal Anti-Myosin (Skeletal, Fast)−Alkaline Phosphatase antibody produced in mouse
clone MY-32, purified from hybridoma cell culture
Synonym(s):
Monoclonal Anti-Myosin (Skeletal, Fast) antibody produced in mouse
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About This Item
Conjugate:
alkaline phosphatase conjugate
Clone:
MY-32, monoclonal
Application:
IF (d)
Species reactivity:
rat, chicken, rabbit, mouse, human, bovine, guinea pig, feline
Citations:
24
Technique(s):
direct immunofluorescence: 1:150 using formalin-fixed, paraffin-embedded human or animal skeletal muscle sections
Uniprot accession no.:
biological source
mouse
conjugate
alkaline phosphatase conjugate
antibody form
purified immunoglobulin
antibody product type
primary antibodies
clone
MY-32, monoclonal
form
buffered aqueous glycerol solution
species reactivity
rat, chicken, rabbit, mouse, human, bovine, guinea pig, feline
technique(s)
direct immunofluorescence: 1:150 using formalin-fixed, paraffin-embedded human or animal skeletal muscle sections
isotype
IgG1
UniProt accession no.
shipped in
wet ice
storage temp.
2-8°C
target post-translational modification
unmodified
Quality Level
Gene Information
human ... MYH1(4619), MYH2(4620)
mouse ... Myh1(17879), Myh2(17882)
rat ... Myh1(287408), Myh2(691644)
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Application
Monoclonal Anti-Myosin (Skeletal, Fast) Alkaline Phosphatase antibody produced in mouse has been used for the detection and localization of skeletal muscle fast and neonatal myosins using immunohistochemistry. It may be used in muscle fiber typing, in studies of in vivo and in vitro muscle development and in the diagnosis of rhabdomyosarcomas.
The level of mysosin (fast) in serum samples from sportsmen with past injury was determined by western blot using monoclonal mouse anti-myosin (skeletal/fast) as the primary antibody at a dilution of 1:90000.
Biochem/physiol Actions
Monoclonal Anti-Myosin (Skeletal, Fast) specifically binds to an epitope in adult rat skeletal myosin heavy chains IIa, IIb and IId (IIx) (in fast-twitch fibers)†. The antibody product is also reactive in humans, bovines, cats, rabbits, mice, guinea pigs and chickens.
Myosin (Skeletal, Fast) is useful not only in fiber typing but also in detection of myogenic tumors. It enables retrospective as well as prospective fiber typing using formalin-fixed, paraffin-embedded material.
Myosins are motor proteins that interact with actin filaments to regulate cell movement. Myosins also modulate cell shape and signaling. Skeletal muscle myosins have three ′fast′ myosin heavy chains isoforms (IIa, IIx, and IIb), a ′slow′ beta-myosin heavy chain isoform and three major myosin light-chain (MLC) isoforms. These myosin isoforms regulate skeletal muscle shortening.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
General description
Localizes an epitope on the myosin heavy chain. Stains the fast (type II) and neonatal isomyosin molecules found in skeletal muscle, but does not stain cardiac muscle, smooth muscle or non-muscle myosin in cultured cells. Does react with human rhabdomyosarcomas.
Monoclonal Anti-Myosin (Skeletal, Fast) (mouse IgG1 isotype) is derived from the hybridoma produced by the fusion of mouse myeloma cells and splenocytes from an immunized mouse.
Immunogen
rabbit muscle myosin.
Physical form
Solution in 0.05 M Tris buffer, pH 8.0, containing 1% bovine serum albumin, 1 mM MgCl2, 50% glycerol, and 15 mM sodium azide.
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Storage Class
10 - Combustible liquids
wgk
WGK 2
Regulatory Information
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Haruhiko Kondoh et al.
Cardiovascular research, 69(2), 466-475 (2006-01-21)
Cell therapy is a promising strategy for ischemic cardiomyopathy. However, the direct injection method has limitations for generalized cell delivery, especially in dilated cardiomyopathy (DCM). We hypothesized that a sheet-shaped myoblast graft would be superior to direct injection for improving
Katy C Liu et al.
Bioarchitecture, 2(5), 158-170 (2012-09-08)
The development of cell-cell junctions was a fundamental step in metazoan evolution, and human health depends on the formation and function of cell junctions. Although it has long been known that actin and conventional myosin have important roles in cell
Glenda Comai et al.
Developmental cell, 31(5), 654-667 (2014-12-10)
The myogenic regulatory genes Myf5, Mrf4, Myod, and Myogenin likely arose by gene duplications during evolution, presumably to address the more demanding requirements of the vertebrate body plan. Two cell lineages were proposed to be regulated independently by Myf5 and
Charles A Stuart et al.
Journal of strength and conditioning research, 31(3), 798-808 (2016-07-06)
Stuart, CA, Lee, ML, South, MA, Howell, MEA, Cartwright, BM, Ramsey, MW, and Stone, MH. Pre-training muscle characteristics of subjects who are obese determine how well exercise training will improve their insulin responsiveness. J Strength Cond Res 31(3): 798-808, 2017-Only
Christopher Taber et al.
Sports (Basel, Switzerland), 6(2) (2018-06-19)
The purpose of this study was to examine the effects of a recovery supplement compared with a placebo on muscle morphology in trained weightlifters. Vastus lateralis and muscle fiber cross sectional area of type I and type II fibers were
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