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A4418

Sigma-Aldrich

Ammonium sulfate

for molecular biology, ≥99.0%

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Synonym(s):
Mascagnite
Linear Formula:
(NH4)2SO4
CAS Number:
7783-20-2
Molecular Weight:
132.14
EC Number:
231-984-1
MDL number:
MFCD00003391
eCl@ss:
38030408
PubChem Substance ID:
24890861
NACRES:
NA.31

grade

for molecular biology

Quality Level

200

Assay

≥99.0%

form

powder

technique(s)

affinity binding assay: suitable
protein purification: suitable

impurities

≤0.005% Insolubles

pH

5.0-6 (25 °C, 132 g/L)

mp

>280 °C (dec.) (lit.)

solubility

H2O: 0.667 g/mL

density

1.77 g/mL at 25 °C (lit.)

anion traces

chloride (Cl-): ≤5 ppm

cation traces

Cu: ≤5 ppm
Fe: ≤5 ppm
Zn: ≤5 ppm
heavy metals (as Pb): ≤2 ppm

absorption

≤0.025 at 280 at 1 M
≤0.03 at 260 at 1 M

suitability

suitable for flow cytometry
suitable for molecular biology

application(s)

metabolomics
microbiology

foreign activity

DNase, RNase, and protease, none detected

SMILES string

N.N.OS(O)(=O)=O

InChI

1S/2H3N.H2O4S/c;;1-5(2,3)4/h2*1H3;(H2,1,2,3,4)

InChI key

BFNBIHQBYMNNAN-UHFFFAOYSA-N

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General description

Ammonium sulfate is a versatile inorganic sulfate salt, plays crucial role in biochemical research, molecular biology, and cell culture. It serves as an indispensable tool for purifying antibodies and precipitating or fractionating proteins. Furthermore, it is instrumental in crystallographic analysis of nucleic acids and proteins and also used as a nitrogen supplement in synthetic laboratory media. Our premium-grade Ammonium Sulfate is meticulously crafted to be DNase, RNase, and Protease-free, ensuring its suitability for cutting-edge research.

Application

Ammonium sulfate helps in quick precipitation of celluar proteins. Ammonium sulfate may be used for the precipitation or fractionation of proteins or for purification of antibodies. It is also useful for crystallographic analysis of nucleic acids and proteins.Ammonium sulfate has been used
  • in the preparation of ′Blue Silver′ colloidal Coomassie stain for proteins.
  • to precipitate the Shiga-like toxin from the culture supernatant.
  • for precipitaion of viral particles from the culture supernatant.

Features and Benefits

  • Heavy metals (as Pb): ≤2 ppm
  • Free from DNase, RNase, and protease

Other Notes

For additional information on our range of Biochemicals, please complete this form.

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Storage Class Code

11 - Combustible Solids

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

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Protein precipitation using ammonium sulfate
P Wingfield
Current Protocols in Protein Science / Editorial Board, John E. Coligan ... [Et Al.], 3 (2001)
Use of ammonium sulphate as a sulphur fertilizer: Implications for ammonia volatilization
David S Powlson, Chris J Dawson
Soil use and management, 38(1), 622-634 (2022)
Assessment of Cell Cycle and Induction of Apoptosis by Shiga-like Toxin Produced by Escherichia coli O157:H7 in T47D Breast Cancer Cells Using Flow Cytometry
Wayan Suardana et al.
Asian Pacific Journal of Cancer Prevention, 23-10, 3247-3252 (2022)
Mohd Baasir Gaisawat et al.
Microorganisms, 8(1) (2020-01-08)
Clostridium (C.) difficile-infection (CDI), a nosocomial gastrointestinal disorder, is of growing concern due to its rapid rise in recent years. Antibiotic therapy of CDI is associated with disrupted metabolic function and altered gut microbiota. The use of probiotics as an
William P Heal et al.
Nature protocols, 7(1), 105-117 (2011-12-24)
A protocol for selective and site-specific enzymatic labeling of proteins is described. The method exploits the protein co-/post-translational modification known as myristoylation, the transfer of myristic acid (a 14-carbon saturated fatty acid) to an N-terminal glycine catalyzed by the enzyme
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