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Merck
CN

A4675

Agar

Type E, suitable for plant cell culture

Synonym(s):

Agar-agar, Gum agar

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About This Item

Linear Formula:
(C12H18O9)n
CAS Number:
9002-18-0
eCl@ss:
42040102
UNSPSC Code:
50405901
NACRES:
NA.21
EC Number:
232-658-1
MDL number:
MFCD00081288

Key Documents

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Product Name

Agar, Type E, suitable for plant cell culture

InChI key

GYYDPBCUIJTIBM-DYOGSRDZSA-N

InChI

1S/C14H24O9/c1-5-8(16)13-11(7(21-5)4-20-13)23-14-10(18)12(19-2)9(17)6(3-15)22-14/h5-18H,3-4H2,1-2H3/t5?,6-,7?,8-,9+,10-,11?,12+,13+,14?/m1/s1

biological source

algae (Rhodophyceae)

type

Type E

form

powder

technique(s)

cell culture | plant: suitable

transition temp

transition temp 32-38 °C (1.5%)

application(s)

agriculture

storage temp.

room temp

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Application

Agar has been used as reagent for the following studies:
  • Plant cell culture studies.
  • As medium supplement for the growth of wild-type Arabidopsis thaliana (ecotype Columbia) and transgenic seeds.
  • For the solidification of culture medium of cork oak somatic embryos.
  • to examine its effect on metal(loid) sensitivity of the Arabidopsis thaliana metal-hypersensitive mutants
Agar suspension culture may be used for the analysis of cell transformation of palyoma virus. It might have been used for incubation of single antibody-forming cell to investigate the plaque formation.
Agar has been used to examine its effect on metal(loid) sensitivity of the Arabidopsis thaliana metal-hypersensitive mutants.

General description

Agar or Type E agar is a thermo-reversible, polysaccharide gelling agent. Structurally it consists of two main fractions about 70% of agarose and 30% of agaropectin. The gelling properties of agar comes from the agarose, the high gel strength backbone consists of a linear polymer of 3-linked β-D-galactose and 4-linked 3,6-anhydro-α-L-galactose (LA) repeating units.

Storage Class

11 - Combustible Solids

wgk

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)

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Certificates of Analysis (COA)

Lot/Batch Number
SLBW5205
SLBV5911
SLBS8479
SLBN1798V
SLBL0898V

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Pere Puigderrajols et al.
Journal of experimental botany, 53(373), 1445-1452 (2002-05-22)
The timing and tissue localization of small heat shock proteins (sHSPs) during cork oak somatic embryo development was investigated under normal growing culture conditions and in response to stress. Western blot analyses using polyclonal antibodies raised against cork oak recombinant
Melissa S LeBlanc et al.
Journal of biotechnology, 163(1), 1-9 (2012-10-31)
Arsenic is a toxic metalloid and recognized carcinogen. Arsenate and arsenite are the most common arsenic species available for uptake by plants. As an inorganic phosphate (Pi) analog, arsenate is acquired by plant roots through endogenous Pi transport systems. Inside
The structure of agar: Part I. Fractionation of a complex mixture of polysaccharides.
Duckworth M and Yaphe W.
Carbohydrate Research, 16(1), 189-197 (1971)
Selection of Agar Reagents for Medium Solidification Is a Critical Factor for Metal (loid) Sensitivity and Ionomic Profiles of Arabidopsis thaliana
Uraguchi S, et al.
Frontiers in Plant Science, 11, 503-503 (2020)
Human bone marrow colony growth in agar-gel.
B L Pike et al.
Journal of cellular physiology, 76(1), 77-84 (1970-08-01)
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