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Merck
CN

A4685

Luminol sodium salt

luminescent, ≥98% (titration), powder and chunks

Synonym(s):

3-Aminophthalhydrazide, 5-Amino-2,3-dihydro-1,4-phthalazinedione

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About This Item

Linear Formula:
C8H6N3O2Na
CAS Number:
20666-12-0
Molecular Weight:
199.14
UNSPSC Code:
12352204
PubChem Substance ID:
24890898
NACRES:
NA.83
MDL number:
MFCD00161491

Key Documents

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Product Name

Luminol sodium salt,

SMILES string

O=C1NN([Na])C(C2=CC=CC(N)=C21)=O

InChI

1S/C8H7N3O2.Na/c9-5-3-1-2-4-6(5)8(13)11-10-7(4)12;/h1-3H,(H4,9,10,11,12,13);/q;+1/p-1

InChI key

RVJVDCVIJCBUTH-UHFFFAOYSA-M

assay

≥98% (titration)

form

powder and chunks

solubility

water: 50 mg/mL, clear to very slightly hazy, colorless to faint red

Quality Level

300

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Application

Luminol sodium salt has been used:

  • as a component of MEBSS buffer in a bioluminescence assay to detect peroxidase activity in whole blood samples
  • to study protein interaction in whole-cell lysates by immunoprecipitation
  • for in vitro reactive oxygen species (ROS) quantification in CD45+ and CD45− cells

Biochem/physiol Actions

Luminol is a redox-sensitive chemiluminescent compound. It emits blue luminescence when the basic aqueous solution of luminol having oxygen atoms come in contact with oxidizing agents like potassium ferricyanide. It is highly stable and is applicable in various fields such as analytical chemistry, metallurgy, biochemistry, and forensics for detecting reactive species. Luminol has the potential to detect defects in neutrophils ex vivo during oxidative metabolism defects such as chronic granulomatous disease and myeloperoxidase deficiency.

Storage Class

11 - Combustible Solids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

dust mask type N95 (US), Eyeshields, Gloves

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Certificates of Analysis (COA)

Lot/Batch Number
SHBS0488
SHBP8858
SHBP9095
SHBN8149
SHBN8148

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Luminol Chemiluminescence-Delay Method for Determination of Copper(II)
Kamidate, Tamio; Ishikawa, Atsushi; Watanabe, Hiroto.
Bulletin of the Chemical Society of Japan, 65, 1591-1591 (1992)
New Developments in Chemiluminescence Research
Gill, Steven, K.
Aldrichimica Acta, 16, 59-59 (1983)
Chemiluminescence detection in analytical chemistry
Lancaster, J. Steven
Endeavour, 16, 194-194 (1992)
Yantu Zhang et al.
Journal of chromatography. A, 1154(1-2), 260-268 (2007-04-24)
A novel method was developed for the simultaneous determination of glucocorticoid residues such as triamcinolone (TR), prednisolone (PR), hydrocortisone (HC), cortisone (CO), methylprednisolone (MP), dexamethasone (DE) and triamcinolone acetonide (TA) by high-performance liquid chromatography (HPLC) coupled with chemiluminescence (CL) detection.
Chemilunimescence of luminol: The chemcial reaction
White E H, et al.
ACS Chemical Biology, 86(5), 940-941 (1964)
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