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A4929

Supelco

N,N′-Bis(acryloyl)cystamine

BioReagent, suitable for electrophoresis

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Synonym(s):
2,2′-(Bisacrylamino)diethyl disulfide, BAC, Bis(2-acrylamidoethyl) disulfide
Empirical Formula (Hill Notation):
C10H16N2O2S2
CAS Number:
60984-57-8
Molecular Weight:
260.38
EC Number:
262-546-8
MDL number:
MFCD00036225
PubChem Substance ID:
24890926
NACRES:
NA.25

product line

BioReagent

Quality Level

200

form

powder

suitability

suitable for electrophoresis

storage temp.

−20°C

SMILES string

C=CC(=O)NCCSSCCNC(=O)C=C

InChI

1S/C10H16N2O2S2/c1-3-9(13)11-5-7-15-16-8-6-12-10(14)4-2/h3-4H,1-2,5-8H2,(H,11,13)(H,12,14)

InChI key

DJVKJGIZQFBFGS-UHFFFAOYSA-N

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Application

BAC is used as a reversible cross-linker for polyacrylamide gels, to simplify isolation of macromolecules separated on the gels. The disulfide linkage can be broken with a suitable reducing agent. A 1-g slice of a 4% (12:1) gel will dissolve in 5 mL of 100 mM DTT in TBE (pH 8.3) in less than 30 min.
BAC is used as a reversible cross-linker for polyacrylamide gels. The disulfide linkage can be broken with a suitable reducing agent. A 12:1 ratio of acrylamide:BAC is recommended for DNA purification. A one gram slice of a 4% gel will dissolve in 5 mL of 100 mM DTT, TBE solution (pH 8.3) in less than 30 min. The pH of 8.3 is optimum for rapid dissolution of the gel.

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

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