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About This Item
CAS Number:
UNSPSC Code:
12352204
NACRES:
NA.54
MDL number:
Specific activity:
≥130 units/mg protein
Biological source:
bovine spleen
biological source
bovine spleen
Quality Segment
type
Type X
form
buffered aqueous glycerol solution
specific activity
≥130 units/mg protein
mol wt
32.5-33 kDa
cation traces
NH4+: ≤10 μg/mg protein
UniProt accession no.
foreign activity
alkaline phosphatase ≤0.01%, guanase ≤0.01%, nucleoside phosphorylase ≤0.1%
storage temp.
2-8°C
Gene Information
cow ... ADA(280712)
General description
Molecular weight: 32.5-33 kDa
pI: 4.85
pI: 4.85
Application
Adenosine Deaminase from bovine spleen has been used in the immobilization on biostrip for stability and catalytic studies.
Adenosine deaminase is useful in various molecular biology assays, such as glycerol release assays . Adenosine deaminase is a potential target for treatments of combined immunodeficiency disease.
Biochem/physiol Actions
Adenosine deaminase is a purine catabolic enzyme which catalyzes the deamination of adenosine and 2′-deoxyadenosine with approximately equal specificity. It is ubiquitous in mammalian tissue, and deficiency in adenosine deaminase has been associated with severe combined immunodeficiency disease.
Physical form
Solution in 50% glycerol, 5 mM potassium phosphate, pH 6.0
Analysis Note
Protein determined by biuret.
Other Notes
One unit will deaminate 1.0 μmole of adenosine to inosine per min at pH 7.5 at 25 °C.
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Storage Class
10 - Combustible liquids
wgk
WGK 2
flash_point_f
Not applicable
flash_point_c
Not applicable
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