A5168
Adenosine Deaminase from bovine spleen
Type IX, ammonium sulfate suspension, 150-200 units/mg protein
Synonym(s):
Adenosine aminohydrolase
biological source
bovine spleen
Quality Level
type
Type IX
form
ammonium sulfate suspension
specific activity
150-200 units/mg protein
mol wt
32.5-33 kDa
UniProt accession no.
foreign activity
5′-AMP deaminase ≤0.01%
alkaline phosphatase ≤0.01%
guanase ≤0.01%
nucleoside phosphorylase ≤0.1%
storage temp.
2-8°C
Gene Information
cow ... ADA(280712)
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General description
Adenosine deaminase/ADA is an enzyme, that is mainly present in lymphocytes and monocytes.
Molecular weight: 32.5-33 kDa
pI: 4.85
pI: 4.85
Application
Adenosine Deaminase from bovine spleen has been used to metabolize adenosine triphosphate (ATP) and adenosine to an inactive form (inosine). It has also been used to as an additive in KRH (Krebs−Ringer solution buffered with Hepes, containing CaCl2) medium in order to remove exogenous adenosine for cyclic adenosine monophosphate (cAMP) assay.
Adenosine deaminase is useful in various molecular biology assays, such as glycerol release assays . Adenosine deaminase is a potential target for treatments of combined immunodeficiency disease.
Biochem/physiol Actions
Adenosine deaminase is a purine catabolic enzyme which catalyzes the deamination of adenosine and 2′-deoxyadenosine with approximately equal specificity. It is ubiquitous in mammalian tissue, and deficiency in adenosine deaminase has been associated with severe combined immunodeficiency disease.
Adenosine deaminase/ADA can be used as an important marker to identify tuberculous pleural effusion. The activity of ADA is usually high in liver disease, tuberculosis, typhoid, infective mononucleosis and several malignancies.
Physical form
Suspension in 3.2 M (NH4)2SO4, 0.01 M potassium phosphate, pH 6.0
Analysis Note
Protein determined by biuret.
Other Notes
One unit will deaminate 1.0 μmole of adenosine to inosine per min at pH 7.5 at 25 °C.
Storage Class Code
10 - Combustible liquids
WGK
WGK 3
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Personal Protective Equipment
dust mask type N95 (US), Eyeshields, Gloves
Regulatory Information
动植物源性产品
低风险生物材料
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American Journal of Physiology. Lung Cellular and Molecular Physiology, 289(3), L489-L496 (2005)
The diagnostic value of adenosine deaminase activity in pulmonary tuberculosis: Comparison between sputum and serum
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Reduced PDE4 expression and activity contributes to enhanced catecholamine-induced cAMP accumulation in adipocytes from FOXC2 transgenic mice
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Articles
Instructions for working with enzymes supplied as ammonium sulfate suspensions
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