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A5168

Adenosine Deaminase from bovine spleen

Type IX, ammonium sulfate suspension, 150-200 units/mg protein

Synonym(s):

Adenosine aminohydrolase

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About This Item

CAS Number:
9026-93-1
UNSPSC Code:
12352204
NACRES:
NA.54
EC Number:
232-817-5
MDL number:
MFCD00081287
EC Number:
3.5.4.4 (BRENDA, IUBMB)
Specific activity:
150-200 units/mg protein
Biological source:
bovine spleen

Key Documents

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Product Name

Adenosine Deaminase from bovine spleen, Type IX, ammonium sulfate suspension, 150-200 units/mg protein

biological source

bovine spleen

type

Type IX

form

ammonium sulfate suspension

specific activity

150-200 units/mg protein

mol wt

32.5-33 kDa

UniProt accession no.

P56658

foreign activity

5′-AMP deaminase ≤0.01%
alkaline phosphatase ≤0.01%
guanase ≤0.01%
nucleoside phosphorylase ≤0.1%

storage temp.

2-8°C

Quality Level

200

Gene Information

cow ... ADA(280712)

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Physical form

Suspension in 3.2 M (NH4)2SO4, 0.01 M potassium phosphate, pH 6.0

General description

Adenosine deaminase/ADA is an enzyme, that is mainly present in lymphocytes and monocytes.
Molecular weight: 32.5-33 kDa

pI: 4.85

Other Notes

One unit will deaminate 1.0 μmole of adenosine to inosine per min at pH 7.5 at 25 °C.

Analysis Note

Protein determined by biuret.

Application

Adenosine Deaminase from bovine spleen has been used to metabolize adenosine triphosphate (ATP) and adenosine to an inactive form (inosine). It has also been used to as an additive in KRH (Krebs−Ringer solution buffered with Hepes, containing CaCl2) medium in order to remove exogenous adenosine for cyclic adenosine monophosphate (cAMP) assay.
Adenosine deaminase is useful in various molecular biology assays, such as glycerol release assays . Adenosine deaminase is a potential target for treatments of combined immunodeficiency disease.

Biochem/physiol Actions

Adenosine deaminase is a purine catabolic enzyme which catalyzes the deamination of adenosine and 2′-deoxyadenosine with approximately equal specificity. It is ubiquitous in mammalian tissue, and deficiency in adenosine deaminase has been associated with severe combined immunodeficiency disease.
Adenosine deaminase/ADA can be used as an important marker to identify tuberculous pleural effusion. The activity of ADA is usually high in liver disease, tuberculosis, typhoid, infective mononucleosis and several malignancies.

Storage Class

10 - Combustible liquids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)

Regulatory Information

低风险生物材料
动植物源性产品
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Certificates of Analysis (COA)

Lot/Batch Number
0000498744
0000498744
0000362925
0000252784
0000362925

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The diagnostic value of adenosine deaminase activity in pulmonary tuberculosis: Comparison between sputum and serum
Atta S, et al.
The Egyptian journal of chest diseases and tuberculosis, 64(1), 103-107 (2015)
Paracrine stimulation of surfactant secretion by extracellular ATP in response to mechanical deformation
Patel A S, et al.
American Journal of Physiology. Lung Cellular and Molecular Physiology, 289(3), L489-L496 (2005)
Reduced PDE4 expression and activity contributes to enhanced catecholamine-induced cAMP accumulation in adipocytes from FOXC2 transgenic mice
Gronning L M, et al.
Febs Letters, 580(17), 4126-4130 (2006)
Ying Cheng et al.
Diabetes, 59(1), 17-25 (2009-10-17)
White adipose tissue (WAT) and brown adipose tissue (BAT) play distinct roles in adaptation to changes in nutrient availability, with WAT serving as an energy store and BAT regulating thermogenesis. We previously showed that mice maintained on a leucine-deficient diet
Leila Antonangelo et al.
Clinics (Sao Paulo, Brazil), 67(11), 1259-1263 (2012-11-28)
Pleural tuberculosis is the most frequently occurring form of extra pulmonary disease in adults. In up to 40% of cases, the lung parenchyma is concomitantly involved, which can have an epidemiological impact. This study aims to evaluate the pleural and
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