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A5268

Alcian Blue 8GX

powder

Synonym(s):

Alcian Blue, Ingrain Blue 1

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About This Item

Empirical Formula (Hill Notation):
C56H68Cl4CuN16S4
CAS Number:
33864-99-2
Molecular Weight:
1298.86
UNSPSC Code:
12171500
NACRES:
NA.47
PubChem Substance ID:
24890962
EC Number:
251-705-7
Colour Index Number:
74240
MDL number:
MFCD00010720
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form

powder

Quality Level

100

composition

Dye content, 45-65%

technique(s)

microbe id | staining: suitable

color

blue

solubility

H2O: 1 mg/mL

εmax

425 at 615-630 nm in water

application(s)

diagnostic assay manufacturing
hematology
histology

storage temp.

room temp

SMILES string

[Cl-].[Cl-].[Cl-].[Cl-].CN(C)C(\SCc1ccc2c3nc(nc4n5[Cu]n6c(nc7nc(nc5c8cc(CS\C(N(C)C)=[N+](\C)C)ccc48)c9ccc(CS\C(N(C)C)=[N+](\C)C)cc79)c%10ccc(CS\C(N(C)C)=[N+](\C)C)cc%10c6n3)c2c1)=[N+](/C)C

InChI

1S/C56H68N16S4.4ClH.Cu/c1-65(2)53(66(3)4)73-29-33-17-21-37-41(25-33)49-57-45(37)62-50-43-27-35(31-75-55(69(9)10)70(11)12)19-23-39(43)47(59-50)64-52-44-28-36(32-76-56(71(13)14)72(15)16)20-24-40(44)48(60-52)63-51-42-26-34(18-22-38(42)46(58-51)61-49)30-74-54(67(5)6)68(7)8;;;;;/h17-28H,29-32H2,1-16H3;4*1H;/q+2;;;;;+2/p-4

InChI key

CKLBXIYTBHXJEH-UHFFFAOYSA-J

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Related Categories

General description

Alcian Blue 8GX, also known as Ingrain Blue 1, is a basic copper phthalocyanine dye whose copper content imparts a blue color. It is tetravalent, cationic, and water-soluble in nature. It forms salt linkages with the acid groups of acid mucopolysaccharides.

Application

  • Alcian Blue 8GX has been used for the staining and precipitation of glycosaminoglycans and as a heteroglycan stain for neutral, sulfated, and phosphated mucopolysaccharides and glycosaminoglycans in tissues such as cartilage and extracellular matrices.
  • It has been employed in the in vivo visualization of intravascular Bonghan ducts and corpuscles in murine models.
  • It has been used to develop a novel histochemical staining method to assess multiple respiratory parameters in various species.

Biochem/physiol Actions

The Alcian blue staining is easily combined with the Periodic acid-Schiff (PAS) reaction. In the tissue, acid mucosubstances are stained selectively and nuclear fast red is commonly used as counterstain. In combination with the PAS reaction, non-substituted polysaccharides, neutral mucopolysaccharides, muco- and glycoproteins, and glycol- and phospholipids can be visualized in addition. An Acetic acid-Alcian blue solution is used for the staining, whereby carboxylated and sulfatic proteoglycans are selectively stained when a pH of 2.5 is applied. The differentiation between carboxyl and sulfate groups is only possible at pH 1.

Features and Benefits

Desired staining patterns can be obtained by manipulating pH and salt concentrations.

Storage Class

11 - Combustible Solids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)

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Certificates of Analysis (COA)

Lot/Batch Number
0000545178
0000545177
0000545167
0000539973
0000545178

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In vivo visualization of Bonghan ducts inside blood vessels of mice by using an Alcian blue staining method
Yoo, et al.
Indian Journal of Experimental Biology, 46 (2008)
Histotechnology: A Self-instructional Text (3rd ed.)
Freida L. Carson & Christa Hladik
Histotechnology: A Self Instructional Text, 4th Edition (2009)
Selective COX-2 inhibition prevents proinflammatory cytokine-induced cartilage damage.
Mastbergen SC, et al.
Rheumatology (Oxford, England), 41, 801-808 (2002)
Exposure of human cartilage tissue to low concentrations of blood for a short period of time leads to prolonged cartilage damage: an in vitro study.
Jansen NW, et al.
Arthritis and Rheumatism, 56, 199-207 (2007)
Alcian Blue and Pyronine Y Histochemical Stains Permit Assessment of Multiple Parameters in Pulmonary Disease Models
Meyerholz, et al.
Veterinary Pathology, 46 (2009)
View All Related Papers

Global Trade Item Number

SKUGTIN
A5268-25G04061833375655
A5268-100G04061833375631
A5268-10G04061833375648

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