A5601
Anti-Arp1α/Centractin antibody produced in rabbit
IgG fraction of antiserum, buffered aqueous solution
Product Name
Anti-Arp1α/Centractin antibody produced in rabbit, IgG fraction of antiserum, buffered aqueous solution
biological source
rabbit
conjugate
unconjugated
antibody form
IgG fraction of antiserum
antibody product type
primary antibodies
clone
polyclonal
form
buffered aqueous solution
mol wt
antigen 45 kDa
species reactivity
mouse, human, rat
technique(s)
indirect immunofluorescence: 1:100 using mouse fibroblasts NIH3T3 cell line
microarray: suitable
western blot: 1:1,000 using rat brain cytosol extract or whole cell extract of human epidermoid carcinoma A431 cell line.
UniProt accession no.
shipped in
dry ice
storage temp.
−20°C
target post-translational modification
unmodified
Gene Information
human ... ACTR1A(10121)
mouse ... Actr1a(54130)
rat ... Actr1a(294010)
Application
Anti-Arp1α/Centractin antibody produced in rabbit has been used in immunoblotting and immunofluorescence staining.
Biochem/physiol Actions
Arp1α or Centractin is an actin-related, ubiquitious protein that forms a part of the dynactin complex and is associated with centrosomes. Studies have reported that centractin interacts with spectrin and subsequently provides a link for dynactin to associate with organelles in cells.
The actin-related proteins (Arp)1α/dynactin complex regulates dyne mediated vesicle movement on microtubules as well as spindle assembly and cell division. Arp1α overexpression in cells results in aberrant spindle morphologies and cell cycle delay at prometaphase, suggesting a possible function of Arp1α/dynactin complex in progression through the prometaphase of mitosis. Expression of mutant Arp1a protein has no effect on mitotic cells, but results in microtubule disruption in interphase cells, by destabilizing the interaction between Arp1a and components of the dynactin complex and nuclear mitotic apparatus protein (NuMA).
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
General description
The actin-related protein Arp1α (centractin, actin-RPV), a member of the superfamily of actin-related proteins (Arp), is the major subunit of the dynactin complex, a key component of the cytoplasmic dynein motor machinery. It has been shown to localize to multiple structures within the cell, including membrane organelles, the centrosome, spindle poles, and spindle pole microtubules during mitosis and prometaphase kinetochores.
Immunogen
synthetic peptide corresponding to the mid-region of human Arp1α/centractin (amino acids 222-240). This sequence is identical in mouse and dog Arp1α/centractin, highly conserved (89% identity) in the α-centractin isoform, and not found in known actin isoforms.
Physical form
Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.
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Storage Class
10 - Combustible liquids
wgk
nwg
flash_point_f
Not applicable
flash_point_c
Not applicable
Regulatory Information
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Antimitotic effect of the retinoid 4-oxo-fenretinide through inhibition of tubulin polymerization: a novel mechanism of retinoid growth-inhibitory activity
Appierto V, et al.
Molecular Cancer Therapeutics, 8(12), 3360-3368 (2009)
Regulation of spindle integrity and mitotic fidelity by BCCIP
Huhn S, et al.
Oncogene, 36(33), 4750-4750 (2017)
S W Clark et al.
Nature, 359(6392), 246-250 (1992-09-17)
Actin is one of the most ubiquitous, abundant and well-conserved proteins of eukaryotes, participating in many crucial cellular processes including the maintenance of cell shape, motility and cell division. Actins from the most divergent sources still share amino-acid identities in
S C Huhn et al.
Oncogene, 36(33), 4750-4766 (2017-04-11)
Centrosomes together with the mitotic spindle ensure the faithful distribution of chromosomes between daughter cells, and spindle orientation is a major determinant of cell fate during tissue regeneration. Spindle defects are not only an impetus of chromosome instability but are
E A Holleran et al.
The Journal of cell biology, 135(6 Pt 2), 1815-1829 (1996-12-01)
Centractin (Arp1), an actin-related protein, is a component of the dynactin complex. To investigate potential functions of the protein, we used transient transfections to overexpress centractin in mammalian cells. We observed that the overexpressed polypeptide formed filamentous structures that were
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