A5852
Alkaline Phosphatase Stop Solution
powder, for ELISA
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About This Item
UNSPSC Code:
12352204
NACRES:
NA.83
Related Categories
Application
Stop solution for Alkaline Phosphatase Red and Blue microwell substrate solutions. Use 50 μl of stop solution per 200 μl of reaction mix. Alkaline Phosphatase Stop Solution has been used in terminating the alkaline phosphatase and nitrotetrazolium blue chloride (NBT)/5-bromo-4-chloro-3-indolyl phosphate (BCIP) reaction on nitrocellulose membrane.
Preparation Note
Reconstitute with 100 mL of water and mix for 30-60 min.
signalword
Warning
hcodes
Hazard Classifications
Eye Irrit. 2 - Skin Irrit. 2 - STOT SE 3
target_organs
Respiratory system
Storage Class
10 - Combustible liquids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
Regulatory Information
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A screen for over-secretion of proteins by yeast based on a dual component cellular phosphatase and immuno-chromogenic stain for exported bacterial alkaline phosphatase reporter.
Nallaseth FS and Anderson S
Microbial cell factories, 12(1), 36-36 (2013)
Articles
NBT-BCIP substrate system aids in western blotting and immunohistological staining, producing a blue-purple insoluble end product.
硝基蓝四唑（NBT）可与碱性磷酸酶底物5-溴-4-氯-3-吲哚基磷酸盐（BCIP）一起用于蛋白印迹和免疫组织染色程序。这些底物系统产生不溶的NBT二甲终产物，其颜色为蓝至紫色，可以通过视觉观察到。
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