A6066
Anti-Rat IgG (whole molecule)–Alkaline Phosphatase antibody produced in rabbit
affinity isolated antibody, buffered aqueous glycerol solution
Synonym(s):
Rabbit Anti-Rat IgG (whole molecule)–AP
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About This Item
Conjugate:
alkaline phosphatase conjugate
Clone:
polyclonal
Application:
ELISA (d), IHC (p), WB
Citations:
20
biological source
rabbit
Quality Level
conjugate
alkaline phosphatase conjugate
antibody form
affinity isolated antibody
antibody product type
secondary antibodies
clone
polyclonal
form
buffered aqueous glycerol solution
technique(s)
direct ELISA: 1:30,000, immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:50-1:100, western blot: 1:30,000-1:60,000
shipped in
wet ice
storage temp.
2-8°C
target post-translational modification
unmodified
Related Categories
General description
An immunoglobulin has two heavy chains and two lights chain connected by disulfide bond. It mainly helps in immune defense. IgG is a glycoprotein and is the major class of immunoglobulin. Rat has five immunoglobulin classes- IgM, IgG, IgA, IgD and IgGE. IgG has four subclasses- IgG1, IgG2a, IgG2b and IgG2c.
IgGs are glycoprotein antibodies that modulate several immune responses. Rat IgGs have four subclasses, namely, IgG1, IgG2a, IgG2b, and IgG2c. Rat IgGs against target proteins are often used as primary antibodies in various research applications. Thus, secondary anti-rat IgG is a useful tool for the analysis of target proteins.
The affinity isolated antibody is obtained from anti-rat IgG antiserum by immunospecific purification, which removes essentially all rabbit serum proteins, including immunoglobulins, which do not specifically bind to rat IgG. Anti-Rat IgG is conjugated to alkaline phosphatase by protein cross linking with 0.2% glutaraldehyde
Identity and purity of the antibody is established by immunoelectrophoresis (IEP) prior to conjugation. Electrophoresis of the product followed by diffusion versus anti-rabbit IgG and anti-rabbit whole serum results in single arcs of precipitation.
Identity and purity of the antibody is established by immunoelectrophoresis (IEP) prior to conjugation. Electrophoresis of the product followed by diffusion versus anti-rabbit IgG and anti-rabbit whole serum results in single arcs of precipitation.
Immunogen
Purified rat IgG
Application
Anti-Rat IgG (whole molecule)−Alkaline Phosphatase antibody produced in rabbit has been used in:
- western blot analysis
- indirect enzyme-linked immunosorbent assay (ELISA)
- RNA immunoprecipitation assay
Biochem/physiol Actions
Binds all rat Igs. Anti-Rat IgG (whole molecule)-Alkaline Phosphatase antibody binds to all rat immunoglobulins.
Immunoglobulin G (IgG) participates in hypersensitivity type II and type III. It helps in opsonization, complement fixation and antibody dependent cell mediated cytotoxicity.
Physical form
Solution in 0.05 M Tris, pH 8.0, containing 1% bovine serum albumin, 1 mM MgCl2, 50% glycerol, and 15 mM sodium azide.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Storage Class
10 - Combustible liquids
flash_point_f
Not applicable
flash_point_c
Not applicable
Regulatory Information
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Toshiki Shimomura et al.
Biophysical journal, 111(6), 1295-1303 (2016-09-23)
In contrast to major flight muscles in the Mecynorrhina torquata beetle, the third axillary (3Ax) muscle is a minor flight muscle that uniquely displays a powerful mechanical function despite its considerably small volume, ∼1/50 that of a major flight muscle.
miR-185 inhibits non-small cell lung cancer cell proliferation and invasion through targeting of SOX9 and regulation of Wnt signaling
Lei Z, et al.
Molecular Medicine Reports, 17(1), 1742-1752 (2018)
Production of recombinant human acid alpha-glucosidase with high-mannose glycans in gnt1 rice for the treatment of Pompe disease
Jung JW, et al.
Journal of Biotechnology, 42-50 (2017)
A beetle flight muscle displays leg muscle microstructure
Shimomura T, et al.
Biophysical Journal, 111(6), 1295-1303 (2016)
Elina G Manzhalii et al.
Digestive diseases (Basel, Switzerland), 40(2), 232-238 (2021-04-12)
The pathophysiology of hepatic encephalopathy (HE) is incompletely understood. It remains elusive how the contributing factors of neuronal ammonia accumulation, cell swelling, and inflammation interact. The objective of this study was to find the correlation between neuronal autoantibody levels and
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Instructions
Global Trade Item Number
| SKU | GTIN |
|---|---|
| A6066-.5ML | 04061837514449 |
| A6066-1ML | 04061837514456 |
| A6066-.25ML | 04061837514432 |
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