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A6103

Aprotinin

recombinant, expressed in Nicotiana (tobacco), ≥5 TIU/mg protein, powder, ≥98% (SDS-PAGE), suitable for inhibition assay

Synonym(s):

BPTI, Basic pancreatic trypsin inhibitor, Kallikrein-trypsin inactivator, Kunitz protease inhibitor

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About This Item

UNSPSC Code:
12352202
NACRES:
NA.77
EC Number:
232-994-9
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Product Name

Aprotinin Nicotiana tobacco, >= 5TIU/mg protein, >= 98 % SDS-PAGE | 9087-70-1, recombinant, expressed in Nicotiana (tobacco), ≥5 TIU/mg protein, ≥98% (SDS-PAGE)

recombinant

expressed in Nicotiana (tobacco)

Quality Level

300

description

recombinant form of thenative bovine-sequence aprotinin purified from plant tissue (tobacco)

product line

BioUltra

assay

≥98% (SDS-PAGE)

form

powder

specific activity

≥5 TIU/mg protein

mol wt

~_6.5 kDa

technique(s)

inhibition assay: suitable

solubility

0.85% sodium chloride: 5 mg/mL

UniProt accession no.

P00974

storage temp.

2-8°C

Gene Information

cow ... PTI(404172)

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Preparation Note

Contains no animal-derived components or impurities, and is manufactured by transient expression of the aprotinin message in RNA (+)-strand tobacco mosaic virus vectors propagated in non-transgenic Nicotiana plants. This is a recombinant form of the native, bovine-sequence aprotinin, which is traditionally isolated from bovine lung by methods involving fractional precipitation, gel filtration, and ion exchange chromatography. Unlike animal-derived aprotinin, this product is isolated and purified from plant tissue by proprietary methods.

Other Notes

One trypsin inhibitor unit (TIU) will decrease the activity of 2 trypsin units by 50% where one trypsin unit will hydrolyze 1.0 μmole of N-α-benzoyl-DL-arginine p-nitroanilide (BAPNA) per min at pH 7.8 at 25 °C.

Storage Class

11 - Combustible Solids

wgk

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)

Regulatory Information

常规特殊物品

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Certificates of Analysis (COA)

Lot/Batch Number
SLCH9755
SLCG0626
SLBW2421
SLBQ7398V
SLBJ8427V

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Xi-Qin Ding et al.
PloS one, 4(10), e7410-e7410 (2009-10-14)
Subretinal delivery of polyethylene glycol-substituted lysine peptide (CK30PEG)-compacted DNA nanoparticles results in efficient gene expression in retinal cells. This work evaluates the ocular safety of compacted DNA nanoparticles. CK30PEG-compacted nanoparticles containing an EGFP expression plasmid were subretinally injected in adult
Allen M Vong et al.
Journal of translational medicine, 9, 101-101 (2011-07-05)
Non Obese Diabetic mice lacking B cells (NOD.Igμ(null) mice) do not develop diabetes despite their susceptible background. Upon reconstitution of B cells using a chimera approach, animals start developing diabetes at 20 weeks of age. We have used the spectratyping
Jerzy Dorosz et al.
Scientific reports, 9(1), 4019-4019 (2019-03-13)
The full length human histone 3 lysine 4 demethylase KDM5B (PLU-1/Jarid1B) has been studied using Hydrogen/Deuterium exchange mass spectrometry, homology modelling, sequence analysis, small angle X-ray scattering and electron microscopy. This first structure on an intact multi-domain Jumonji histone demethylase
Radmila Hrdlicková et al.
Molecular and cellular biology, 29(3), 929-941 (2008-12-03)
Telomerase activity is downregulated in somatic cells but is upregulated during the activation of cells of the immune system. The mechanism of this reactivation is not well understood. In this study, we demonstrated that interferon regulatory factor 4 (IRF-4) and
E L Leung et al.
British journal of cancer, 98(11), 1803-1809 (2008-05-29)
The present study determines if (1) basal protein levels of nitric oxide (NO) synthases (eNOS, iNOS, and nNOS) are different in cisplatin-sensitive (OV2008) and counterpart cisplatin-resistant (C13(*)) human ovarian cancer cells, (2) cisplatin alters NOS levels, (3) NO donor causes
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Articles

ReadyShield Phosphatase and Protease Inhibitor Cocktail FAQ

ReadyShield® phosphatase and protease inhibitor cocktail FAQ for sample protection in a variety of cell types and tissue extracts, including mammalian, plant, and microbial samples. Our ReadyShield® Protease Inhibitor Cocktail is a non-freezing solution that contains inhibitors with a broad specificity for serine, cysteine, acid proteases and aminopeptidases.

Aprotinin

While aprotinin and bovine pancreatic trypsin inhibitor (BPTI) are the same protein sequence, the term aprotinin is typically used when describing the protein derived from bovine lung.

Protocols

Enzymatic Assay of Aprotinin

Objective: To standardize a procedure for the enzymatic assay of Aprotinin.

抑肽酶酶活测定

抑肽酶是一种牛胰蛋白酶抑制剂。在测定抑肽酶活性时，该测定使用了在405 nm下的连续分光光度法进行测定。

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Global Trade Item Number

SKUGTIN
A6103-100MG04061833377055
A6103-1MG04061833377079
A6103-25MG04061833377086

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