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About This Item
Empirical Formula (Hill Notation):
C14H14ClN3S
CAS Number:
Molecular Weight:
291.80
UNSPSC Code:
12171500
NACRES:
NA.47
PubChem Substance ID:
EC Number:
208-510-7
MDL number:
Colour Index Number:
52005
Beilstein/REAXYS Number:
3922287
Product Name
Azure A chloride, Dye content ≥70 %
form
powder or crystals
Quality Segment
composition
Dye content, ≥70%
color
green to very dark green
mp
290 °C (dec.) (lit.)
solubility
water: 1 mg/mL
application(s)
diagnostic assay manufacturing
hematology
histology
storage temp.
room temp
SMILES string
NC1=CC(SC(C2=N3)=CC(C=C2)=[N+](C)C)=C3C=C1.[Cl-]
InChI
1S/C14H13N3S.ClH/c1-17(2)10-4-6-12-14(8-10)18-13-7-9(15)3-5-11(13)16-12;/h3-8,15H,1-2H3;1H
InChI key
NALREUIWICQLPS-UHFFFAOYSA-N
Application
Azure A chloride has been used for the staining of polyacrylamide gel to analyse the bovine lung heparin digestion by heparinase I. It has been used for the determination of the sulphated groups content in polysaccharides.
Biochem/physiol Actions
Azure A is a basic cationic dye. The combination of azure A and silver staining is used for the quantification of glycosaminoglycans in agarose gels. It is also used for the staining of chromosomes in the nucleus. It is a main component of the azure B/polychromed methylene blue – eosin stains family, also referred to as the Romanowsky stain.
Disclaimer
Handle and store under argon. Glove bag item.
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Storage Class
11 - Combustible Solids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, type N95 (US)
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Related Content
Generating heparan sulfate saccharide libraries for glycomics applications.
Powell AK, et al.
Nature Protocols, 5, 821-833 (2010)
Quantification and characterization of glycosaminoglycans at the nanogram level by a combined azure A-silver staining in agarose gels.
van de Lest CH, et al.
Analytical Biochemistry, 221, 356-361 (1994)
Biochemical and antiviral activities of enzymatic hydrolysates from different invasive French seaweeds.
Hardouin K, et al.
Journal of Applied Phycology, 26.2, 1029-1042 (2014)
Global Trade Item Number
| SKU | GTIN |
|---|---|
| A6270-50G | 04061835345786 |
| A6270-10G | 04061835345779 |