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Merck
CN

A6751

N-Acetyl-L-tyrosine ethyl ester monohydrate

≥98%, suitable for ligand binding assays

Synonym(s):

ATEE

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About This Item

Empirical Formula (Hill Notation):
C13H17NO4 · H2O
CAS Number:
Molecular Weight:
269.29
PubChem Substance ID:
eCl@ss:
32160406
UNSPSC Code:
12352209
NACRES:
NA.26
EC Number:
212-663-5
MDL number:
Beilstein/REAXYS Number:
2217900
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Product Name

N-Acetyl-L-tyrosine ethyl ester monohydrate,

InChI

1S/C13H17NO4/c1-3-18-13(17)12(14-9(2)15)8-10-4-6-11(16)7-5-10/h4-7,12,16H,3,8H2,1-2H3,(H,14,15)/t12-/m0/s1

InChI key

SKAWDTAMLOJQNK-LBPRGKRZSA-N

SMILES string

CCOC(=O)[C@H](Cc1ccc(O)cc1)NC(C)=O

assay

≥98%

form

powder

technique(s)

ligand binding assay: suitable

color

white

storage temp.

−20°C

Quality Level

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Application

N-Acetyl-L-tyrosine ethyl ester monohydrate has been used:
  • as a substrate in transesterification reactions with 1 M propan-1-ol catalyzed by Carlsberg protease protein-coated microcrystals (PCMC)
  • to test the performance of subtilisin Carlsberg protein-coated microcrystals (PCMC)
  • as a substrate for cross-linked crystals (CLECs) of subtilisin activity screening

Biochem/physiol Actions

N-Acetyl-L-tyrosine ethyl ester is an N-terminal and C-terminal protected L-tyrosine that is used in crosslinking studies and as a substrate for the detection, differentiation and/or characterization various proteases and esterases.

General description

Substrate for chymotrypsin

Storage Class

11 - Combustible Solids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)


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Sandra M Arriaga et al.
Clinical biochemistry, 42(9), 919-921 (2009-01-20)
To evaluate if unconjugated bilirubin (UB) inhibits C1 esterase activity. Esterase activity was evaluated by C1-mediated hydrolysis of N-acetyl-L-tyrosine ethyl ester, and binding of UB to C1r and C1s was assessed by dot-blot analysis. UB inhibited C1 enzymatic activity. C1r
Operational stability of subtilisin CLECs in organic solvents in repeated batch and in continuous operation
Fernandes JFA, et al.
Biochemical Engineering Journal, 24(1) (24)
Michaela Kreiner et al.
Biotechnology and bioengineering, 87(1), 24-33 (2004-06-24)
Recently, we reported a new high-activity biocatalyst for use in organic media termed protein-coated microcrystals (PCMC) (Kreiner et al. [2001] Chem Commun 12:1096-1097). These novel particles consist of water-soluble micron-sized crystalline particles coated with the given biocatalyst(s) and are prepared
A study of the intermolecular interactions of tolmetin/N-acetyl-L-tyrosine ethyl ester complex.
Tito A, Jimenez-Lopez C, et al.
Spectrochimica Acta Part A: Molecular Spectroscopy, 72, 1000-1006 (2009)
Alicja Janik et al.
Acta crystallographica. Section C, Crystal structure communications, 63(Pt 10), o572-o575 (2007-10-06)
The crystal structure of a protected L-tyrosine, namely N-acetyl-L-tyrosine methyl ester monohydrate, C(12)H(15)NO(4).H(2)O, was determined at both 293 (2) and 123 (2) K. The structure exhibits a network of O-H...O and N-H...O hydrogen bonds, in which the water molecule plays

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