HydroMatrix Peptide Cell Culture Scaffold

HydroMatrix has been shown to support improved growth and migration in the proliferation of many cell types, including neural stem cells, neurons, glia, astrocytes, fibroblasts, and keratinocytes.

This product is supplied as a lyophilized powder. A 1% (w/v) stock solution at 10 mg/mL can be prepared with addition of sterile water, resulting in a rigid gel. A 0.5% (w/v) solution will lead to a softer gel that is more suitable for cell migration and angiogenesis. Lower concentrations in the range of 0.15%-0.25% are more suitable for many cell types including neurons. In aqueous solution, HydroMatrix has a pH of 2.5 and should not be added directly to cells without prior addition of a buffer or medium. Once the hydrogel has formed, do not attempt to mix the gel any further, as this risks destroying the hydrogel integrity. Manipulation of the cells should be carefully performed to avoid disruption of the scaffold.

HydroMatrix is a peptide nanofiber 3-dimensional scaffold that promotes cell growth and migration. It utilizes specific peptides that self-assemble from fluid precursors into highly crosslinked peptide hydrogels when presented with increased temperatures or ionic strengths. By inducing a rapid transformation from solution to gel, a peptide nanofiber scaffold is generated. Adjusting the concentration of the HydroMatrix solution, researchers can control the flexibility of the 3-D architecture to meet their application′s specific needs.

The lyophilized product at -20°C and is stable for many years at this temperature. The hydrogel should be stored at -70°C to maximize its active span. The 1% (w/v) stock solution should be stored -20°C and is stable for 3-6 months.