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A7921

Agar

Name: Agar
Brand: SIGMA

suitable for plant cell culture, powder

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Synonym(s):

Agar-agar, Gum agar

Linear Formula:

(C₁₂H₁₈O₉)_n

CAS Number:

9002-18-0

EC Number:

232-658-1

MDL number:

MFCD00081288

eCl@ss:

42040102

NACRES:

NA.21

biological source

algae (Rhodophyceae)

Quality Level

200

form

powder

technique(s)

cell culture | plant: suitable

transition temp

congealing temperature 30-35 °C

application(s)

agriculture

storage temp.

room temp

InChI

1S/C14H24O9/c1-5-8(16)13-11(7(21-5)4-20-13)23-14-10(18)12(19-2)9(17)6(3-15)22-14/h5-18H,3-4H2,1-2H3/t5?,6-,7?,8-,9+,10-,11?,12+,13+,14?/m1/s1

InChI key

GYYDPBCUIJTIBM-DYOGSRDZSA-N

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Microbial Culture Media Raw Materials

Plant Culture Media

General description

Agar contains agarose and agaropectin, with agarose being the major gelling heteropolysaccharide. Agarose contains β-d-galactose and 3,6-anhydro-α-l-galactose, linked by glycosidic bonds β(1-4) called the neoagarobiose. It also has agarobiose with same disaccharide units with α(1-3) linkage. The major source of agar is the cell wall of seaweed from Rhodophyceae family.

Application

Agar has been used as a component of EDMm (modified version of Eucalyptus dunnii basal medium (EDM)) for development of shoots of Eucalyptus dunnii clones. It has also been used as a component of Murashige and Skoog medium for in vitro culture of Strain TC09 of Cladosporium sphaerospermum.

Agar has been used as a component

of Gamborg′s B5 regeneration medium
of Murashige and skoog (MS) medium for the germination of Hypericum triquetrifolium Turra seeds
in shoot inducing and naphthalene acetic acid pretreatment medium for the regeneration of Arabidopsis thaliana seedlings

Biochem/physiol Actions

Agar is majorly used as microbial solid culture media. It is used in the range of 0.5 to 1.0% (w/v) to solidify plant tissue culture media. It is essential for in vitro root development.

Other Notes

Purified for research and protoplast culture.

Preparation Note

Typical working concentration: 6-12 g/L.

Storage Class Code

11 - Combustible Solids

WGK

WGK 1

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

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Plant Tissue Culture, Development, and Biotechnology, 21(11), 1577-1577 (2011)

Exposure in vitro to an Environmentally Isolated Strain TC09 of Cladosporium sphaerospermum Triggers Plant Growth Promotion, Early Flowering, and Fruit Yield Increase.

Zhijian T Li et al.

Frontiers in plant science, 9, 1959-1959 (2019-02-19)

A growing number of bacteria and fungi have been found to promote plant growth through mutualistic interactions involving elements such as volatile organic compounds (VOCs). Here, we report the identification of an environmentally isolated strain of Cladosporium sphaerospermum (herein named

Study of the influence of wavelengths and intensities of LEDs on the growth, photosynthetic pigment, and volatile compounds production of Lippia rotundifolia Cham in vitro.

Bety Shiue de Hsie et al.

Journal of photochemistry and photobiology. B, Biology, 198, 111577-111577 (2019-08-04)

Lippia rotundifolia Cham. is in the family Verbenaceae and is endemic to the Cerrado. This species is aromatic and characterized by the presence of glandular trichomes on its leaves that are rich in monoterpenes. The objective of this study was

Exploration of movement artefacts in handheld laser speckle contrast perfusion imaging.

Ata Chizari et al.

Biomedical optics express, 11(5), 2352-2365 (2020-06-06)

Functional performance of handheld laser speckle contrast imaging (LSCI) is compromised by movement artefacts. Here we quantify the movements of a handheld LSCI system employing electromagnetic (EM) tracking and measure the applied translational, tilt and on-surface laser beam speeds. By

Protocol: a method to study the direct reprogramming of lateral root primordia to fertile shoots

Kareem A, et al.

Plant methods, 12(1), 27-27 (2016)

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