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A7921

Agar

Name: Agar
Brand: SIGMA

suitable for plant cell culture, powder

Synonym(s):

Agar-agar, Gum agar

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About This Item

Linear Formula:

(C₁₂H₁₈O₉)_n

CAS Number:

9002-18-0

UNSPSC Code:

50405901

eCl@ss:

42040102

EC Number:

232-658-1

NACRES:

NA.21

MDL number:

MFCD00081288

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Properties

biological source

algae (Rhodophyceae)

Quality Segment

200

form

powder

technique(s)

cell culture | plant: suitable

transition temp

congealing temperature 30-35 °C

application(s)

agriculture
food and beverages
life science and biopharma

storage temp.

room temp

SMILES string

O1[C@@H]([C@@H]([C@@H]([C@H](C1OC2[C@H]3OCC2OC([C@H]3O)C)O)OC)O)CO

InChI

1S/C14H24O9/c1-5-8(16)13-11(7(21-5)4-20-13)23-14-10(18)12(19-2)9(17)6(3-15)22-14/h5-18H,3-4H2,1-2H3/t5?,6-,7?,8-,9+,10-,11?,12+,13+,14?/m1/s1

InChI key

GYYDPBCUIJTIBM-DYOGSRDZSA-N

Description

General description

Agar contains agarose and agaropectin, with agarose being the major gelling heteropolysaccharide. Agarose contains β-d-galactose and 3,6-anhydro-α-l-galactose, linked by glycosidic bonds β(1-4) called the neoagarobiose. It also has agarobiose with same disaccharide units with α(1-3) linkage. The major source of agar is the cell wall of seaweed from Rhodophyceae family.

Application

Agar has been used as a component

of Gamborg′s B5 regeneration medium
of Murashige and skoog (MS) medium for the germination of Hypericum triquetrifolium Turra seeds
in shoot inducing and naphthalene acetic acid pretreatment medium for the regeneration of Arabidopsis thaliana seedlings

Agar has been used as a component of EDMm (modified version of Eucalyptus dunnii basal medium (EDM)) for development of shoots of Eucalyptus dunnii clones. It has also been used as a component of Murashige and Skoog medium for in vitro culture of Strain TC09 of Cladosporium sphaerospermum.

Biochem/physiol Actions

Agar is majorly used as microbial solid culture media. It is used in the range of 0.5 to 1.0% (w/v) to solidify plant tissue culture media. It is essential for in vitro root development.

Preparation Note

Typical working concentration: 6-12 g/L.

Other Notes

Purified for research and protoplast culture.

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Storage Class

11 - Combustible Solids

wgk

WGK 1

ppe

Eyeshields, Gloves, type N95 (US)

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