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Merck
CN

A7921

Agar

suitable for plant cell culture, powder

Synonym(s):

Agar-agar, Gum agar

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About This Item

Linear Formula:
(C12H18O9)n
CAS Number:
9002-18-0
UNSPSC Code:
50405901
eCl@ss:
42040102
EC Number:
232-658-1
NACRES:
NA.21
MDL number:
MFCD00081288

Key Documents

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SMILES string

O1[C@@H]([C@@H]([C@@H]([C@H](C1OC2[C@H]3OCC2OC([C@H]3O)C)O)OC)O)CO

InChI

1S/C14H24O9/c1-5-8(16)13-11(7(21-5)4-20-13)23-14-10(18)12(19-2)9(17)6(3-15)22-14/h5-18H,3-4H2,1-2H3/t5?,6-,7?,8-,9+,10-,11?,12+,13+,14?/m1/s1

InChI key

GYYDPBCUIJTIBM-DYOGSRDZSA-N

biological source

algae (Rhodophyceae)

form

powder

technique(s)

cell culture | plant: suitable

transition temp

congealing temperature 30-35 °C

storage temp.

room temp

Quality Level

200

application(s)

agriculture
food and beverages
life science and biopharma

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General description

Agar contains agarose and agaropectin, with agarose being the major gelling heteropolysaccharide. Agarose contains β-d-galactose and 3,6-anhydro-α-l-galactose, linked by glycosidic bonds β(1-4) called the neoagarobiose. It also has agarobiose with same disaccharide units with α(1-3) linkage. The major source of agar is the cell wall of seaweed from Rhodophyceae family.

Application

Agar has been used as a component of EDMm (modified version of Eucalyptus dunnii basal medium (EDM)) for development of shoots of Eucalyptus dunnii clones. It has also been used as a component of Murashige and Skoog medium for in vitro culture of Strain TC09 of Cladosporium sphaerospermum.
Agar has been used as a component
  • of Gamborg′s B5 regeneration medium
  • of Murashige and skoog (MS) medium for the germination of Hypericum triquetrifolium Turra seeds
  • in shoot inducing and naphthalene acetic acid pretreatment medium for the regeneration of Arabidopsis thaliana seedlings

Biochem/physiol Actions

Agar is majorly used as microbial solid culture media. It is used in the range of 0.5 to 1.0% (w/v) to solidify plant tissue culture media. It is essential for in vitro root development.

Preparation Note

Typical working concentration: 6-12 g/L.

Other Notes

Purified for research and protoplast culture.

Storage Class

11 - Combustible Solids

wgk

WGK 1

ppe

Eyeshields, Gloves, type N95 (US)

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Certificates of Analysis (COA)

Lot/Batch Number
BCCN4967
BCCM8890
BCCM1345
BCCM1365
BCCL3112

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Plant Tissue Culture, Development, and Biotechnology, 21(11), 1577-1577 (2011)
Zhijian T Li et al.
Frontiers in plant science, 9, 1959-1959 (2019-02-19)
A growing number of bacteria and fungi have been found to promote plant growth through mutualistic interactions involving elements such as volatile organic compounds (VOCs). Here, we report the identification of an environmentally isolated strain of Cladosporium sphaerospermum (herein named
Ata Chizari et al.
Biomedical optics express, 11(5), 2352-2365 (2020-06-06)
Functional performance of handheld laser speckle contrast imaging (LSCI) is compromised by movement artefacts. Here we quantify the movements of a handheld LSCI system employing electromagnetic (EM) tracking and measure the applied translational, tilt and on-surface laser beam speeds. By
Protocol: a method to study the direct reprogramming of lateral root primordia to fertile shoots
Kareem A, et al.
Plant methods, 12(1), 27-27 (2016)
Bety Shiue de Hsie et al.
Journal of photochemistry and photobiology. B, Biology, 198, 111577-111577 (2019-08-04)
Lippia rotundifolia Cham. is in the family Verbenaceae and is endemic to the Cerrado. This species is aromatic and characterized by the presence of glandular trichomes on its leaves that are rich in monoterpenes. The objective of this study was
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