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A8353

Anti-hABH3 antibody, Mouse monoclonal

Name: Anti-hABH3 antibody, Mouse monoclonal
Brand: SIGMA

clone hABH3-99, purified from hybridoma cell culture

Synonym(s):

Anti-Human AlkB Homologue 3, Monoclonal Anti-hABH3 antibody produced in mouse

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About This Item

UNSPSC Code:

12352203

NACRES:

NA.41

MDL number:

MFCD06412252

Conjugate:

unconjugated

Clone:

hABH3-99, monoclonal

Application:

ARR, ELISA (i), IHC, IP, WB

Citations:

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biological source

mouse

Quality Level

200

conjugate

unconjugated

antibody form

purified immunoglobulin

antibody product type

primary antibodies

clone

hABH3-99, monoclonal

form

buffered aqueous solution

mol wt

antigen ~33 kDa

species reactivity

rat, hamster, monkey, bovine, canine, human, mouse

technique(s)

immunohistochemistry: suitable, immunoprecipitation (IP): suitable, indirect ELISA: suitable, microarray: suitable, western blot: 1-2 μg/mL using total cell extract of 293T cells

isotype

IgG1

UniProt accession no.

Q96Q83

shipped in

dry ice

storage temp.

−20°C

target post-translational modification

unmodified

Gene Information

Monkey ... ALKBH3(715921)
human ... ALKBH3(221120)
mouse ... Alkbh3(69113)
rat ... Alkbh3(362169)

General description

Human alkB homolog 3, alpha-ketoglutarate dependent dioxygenase (hABH3) is mainly found in the nucleoplasm and cytoplasm. Anti-hABH3 antibody, Mouse monoclonal (mouse IgG1 isotype) is derived from the hABH3-99 hybridoma produced by the fusion of mouse myeloma cells (NS1 cells) and splenocytes from BALB/c mice immunized with hABH3, conjugated to keyhole limpet hemocyanin (KLH).

Application

Mouse Monoclonal Anti-hABH3 has been used in:
western blot
immunohistochemistry
immunoprecipitation
indirect enzyme linked immunosorbent assay (ELISA)

Mouse Monoclonal Anti-hABH3 can be used for western blot (1-2μg/mL), immunohistochemistry, immunoprecipitation, indirect ELISA and microarray studies.

Biochem/physiol Actions

Human alkB homolog 3, α-ketoglutarate dependent dioxygenase (hABH3) is involved in the repair of alkylated RNA. This antibody serves as an important tool for studying the regulation of DNA/RNA repair enzymes.

Mouse Monoclonal Anti-hABH3 binds to human, monkey, bovine, dog, rat, hamster, and mouse hABH3. It does not recognize hABH1 or hABH2. The antibody epitope resides within amino acids 2-16 of human hABH3.

hABH3 is a Fe(II)-dependent dioxygenase involved in the repair of alkylation-induced damage in DNA. hABH3 mutations have been analysed in pediatric brain tumors.
Mouse Monoclonal Anti-hABH3 binds to human, monkey, bovine, dog, rat, hamster, and mouse hABH3. It does not recognize hABH1 or hABH2. The antibody epitope resides within amino acids 2-16 of human hABH3.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, and 15 mM sodium azide.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Storage Class

12 - Non Combustible Liquids

wgk

nwg

flash_point_f

Not applicable

flash_point_c

Not applicable

Regulatory Information

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Human and bacterial oxidative demethylases repair alkylation damage in both RNA and DNA

Aas PA, et al.

Nature, 421(6925), 859-859 (2003)

Pediatric brain tumors: mutations of two dioxygenases (hABH2 and hABH3) that directly repair alkylation damage.

Valentina Cetica et al.

Journal of neuro-oncology, 94(2), 195-201 (2009-03-18)

Alkylating agents, commonly used for brain tumor therapy, induce DNA and RNA lesions that, if not repaired, drive cells to apoptosis. Thus, cellular mechanisms that are responsible for nucleic acid repair are possibly involved in drug resistance. This work analyzes

Antibody-based proteomics for esophageal cancer: Identification of proteins in the nuclear factor-kappaB pathway and mitotic checkpoint.

Norihisa Uemura et al.

Cancer science, 100(9), 1612-1622 (2009-06-30)

To identify the molecular background of esophageal cancer, we conducted a proteomics study using an antibody microarray consisting of 725 antibodies and surgical specimens from three cases. The microarray analysis identified 24 proteins with aberrant expression in esophageal cancer compared

Global Trade Item Number

SKU	GTIN
A8353-200UL	04061837789397

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