A8542
Anti-Human IgG (Fab specific)−Alkaline Phosphatase antibody produced in goat
affinity isolated antibody, buffered aqueous solution
Synonym(s):
Goat Anti-Human IgG (Fab specific)−AP
Product Name
Anti-Human IgG (Fab specific)−Alkaline Phosphatase antibody produced in goat, affinity isolated antibody, buffered aqueous solution
biological source
goat
conjugate
alkaline phosphatase conjugate
antibody form
affinity isolated antibody
antibody product type
secondary antibodies
clone
polyclonal
form
buffered aqueous solution
technique(s)
direct ELISA: 1:40,000
dot blot: 1:30,000
immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:50
western blot (chemiluminescent): 1:30,000
shipped in
wet ice
storage temp.
2-8°C
target post-translational modification
unmodified
Quality Level
Related Categories
Biochem/physiol Actions
Immunoglobulin G (IgG) participates in hypersensitivity type II and type III.
Binds all human Igs.
Provides reduced background staining with mouse or rat samples.
Provides reduced background staining with mouse or rat samples.
Application
Anti-Human IgG (Fab specific)−Alkaline Phosphatase antibody produced in goat has been used in enzyme-linked immunosorbent assay (ELISA) and chemiluminescent ELISA.
Nuclear proteins isolated from primary neuronal cells were analyzed by western blot using alkaline phosphatase-conjugated goat anti-human Fab specific as the secondary antibody.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
General description
Antiserum is developed in goat using purified human IgG Fab fragment as the immunogen. Antibody is isolated from goat anti-human IgG antiserum by immunospecific purification which removes essentially all goat serum proteins, including immunoglobulins, which do not specifically bind to the Fab fragment of human IgG. Goat anti-human IgG is conjugated to Alkaline Phosphatase by protein cross linking with 0.2% glutaraldehyde
Specificity of the Alkaline Phosphatase Conjugated Anti-Human IgG is determined by Enzyme Linked Immunosorbent Assay (ELISA). Cross reactivity of the antibody-conjugate is also determined by ELISA
Identity and purity of the antibody is established by immunoelectrophoresis (IEP), prior to conjugation. Electrophoresis of the antibody preparation followed by diffusion versus anti-goat IgG and anti-goat whole serum result in single arcs of precipitation.
Specificity of the Alkaline Phosphatase Conjugated Anti-Human IgG is determined by Enzyme Linked Immunosorbent Assay (ELISA). Cross reactivity of the antibody-conjugate is also determined by ELISA
Identity and purity of the antibody is established by immunoelectrophoresis (IEP), prior to conjugation. Electrophoresis of the antibody preparation followed by diffusion versus anti-goat IgG and anti-goat whole serum result in single arcs of precipitation.
Immunoglobulin G (IgG) belongs to the immunoglobulin family and is a widely expressed serum antibody. Immunoglobulin have two heavy chains and two light chains connected by a disulfide bond. It is a glycoprotein and mainly helps in immune defense. IgG is usually found as a monomer. IgG is further subdivided into four classes namely, IgG1, IgG2, IgG3, and IgG4. IgG antibody subtype is the most abundant of serum immunoglobulins of the immune system. About 70 % of the total immunoglobulin consists of IgG.
The conjugate is specific for human IgG and human IgG Fab fragment. The conjugate shows no reactivity with human IgG Fc fragment, mouse IgG or rat IgG.
Immunogen
Developed in goat using purified human IgG Fab fragment
Other Notes
No cross-reaction with mouse and rat IgG.
Physical form
Solution in 0.05 M Tris, pH 8.0, containing 1% bovine serum albumin, 1 mM MgCl2 and 15 mM sodium azide
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Storage Class
12 - Non Combustible Liquids
wgk
WGK 2
flash_point_f
Not applicable
flash_point_c
Not applicable
Regulatory Information
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Christoph J Slavetinsky et al.
eLife, 11 (2022-01-20)
The pandemic of antibiotic resistance represents a major human health threat demanding new antimicrobial strategies. Multiple peptide resistance factor (MprF) is the synthase and flippase of the phospholipid lysyl-phosphatidylglycerol that increases virulence and resistance of methicillin-resistant Staphylococcus aureus (MRSA) and
Pharmacokinetics and immunologic consequences of exposing macaques to purified homologous butyrylcholinesterase
Rosenberg Y, et al.
Life Sciences, 72(2), 125-134 (2002)
Gerald S Baron et al.
The Journal of biological chemistry, 278(17), 14883-14892 (2003-02-21)
Prion protein (PrP) is usually bound to membranes by a glycosylphosphatidylinositol (GPI) anchor that associates with detergent-resistant membranes, or rafts. To examine the effect of membrane association on the interaction between the normal protease-sensitive PrP isoform (PrP-sen) and the protease-resistant
Evaluation of a latex agglutination kit for the detection of human antibodies to the lipopolysaccharide of Escherichia coli O157, following infection with verocytotoxin-producing E. coli O157
Chart H
Letters in Applied Microbiology, 29(6), 434-436 (1999)
Michelle R Ugolini-Lopes et al.
Advances in rheumatology (London, England), 59(1), 19-19 (2019-05-16)
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