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Merck
CN

A8781

β-Amylase from sweet potato

greener alternative

Synonym(s):

1,4-α-D-Glucan maltohydrolase

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About This Item

CAS Number:
9000-91-3
UNSPSC Code:
41106514
NACRES:
NA.56
EC Number:
232-566-1
MDL number:
MFCD00081391
EC Number:
3.2.1.2 (BRENDA, IUBMB)

Key Documents

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form

powder

mol wt

~200,000

packaging

vial of 15 mg

greener alternative product score

old score: 57
new score: 1
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greener alternative product characteristics

Waste Prevention
Safer Solvents and Auxiliaries
Design for Energy Efficiency
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sustainability

Greener Alternative Product

greener alternative category

Re-engineered,

storage temp.

−20°C

Quality Level

100

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Application

β-Amylase from sweet potato has been used:
  • as a molecular weight marker in blue native polyacrylamide gel electrophoresis (PAGE)
  • as a standard for calibration curve preparation in size exclusion chromatography
  • as a model protein for small-angle X-ray analysis (SAXs)

Biochem/physiol Actions

β-Amylase acts on the polysaccharide α(1,4) glycosidic linkages and releases maltose present in the non-reducing ends. Due to its stability, β-Amylase from sweet potato is used in the immobilized enzyme preparation in combination with agarose beads.

General description

β-Amylase from sweet potato is a tetrameric enzyme with each monomer comprising (α/β) core domain and a C-terminal loop region. It belongs to the glycoside hydrolase family GH14.
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pictograms

Health hazard
GHS08

signalword

Danger

hcodes

H334

Hazard Classifications

Resp. Sens. 1

Storage Class

11 - Combustible Solids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

dust mask type N95 (US), Eyeshields, Faceshields, Gloves

Regulatory Information

动植物源性产品
常规特殊物品
This item has

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Certificates of Analysis (COA)

Lot/Batch Number
0000290697
0000290697
SLCQ9616
SLCQ9616
SLCP3236

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Purification of staphylococcal protein A by affinity precipitation using a reversibly soluble-insoluble polymer with human IgG as a ligand
Taniguchi, M. et al.
Journal of Fermentation and Bioengineering, 68, 32-36 (1989)
Determination of hydrodynamic radius of proteins by size exclusion chromatography
LaVV, et al.
Bio-protocol, 7(8), 14-Jan-14-Jan (2017)
Luca Costa et al.
BMC structural biology, 16(1), 18-18 (2016-10-30)
Synchrotron radiation facilities are pillars of modern structural biology. Small-Angle X-ray scattering performed at synchrotron sources is often used to characterize the shape of biological macromolecules. A major challenge with high-energy X-ray beam on such macromolecules is the perturbation of
Khalilah G Reddie et al.
Molecular bioSystems, 4(6), 521-531 (2008-05-22)
Oxidation of the thiol functional group in cysteine (Cys-SH) to sulfenic (Cys-SOH), sulfinic (Cys-SO2H) and sulfonic acids (Cys-SO3H) is emerging as an important post-translational modification that can activate or deactivate the function of many proteins. Changes in thiol oxidation state
S Teotia et al.
Enzyme and microbial technology, 28(9-10), 792-795 (2001-06-09)
beta-amylases are used in production of maltose syrup. It is shown that sweet potato beta-amylase can be purified by affinity precipitation with alginate with 80% activity yield and 44 fold purification. SDS-PAGE of the purified protein showed a single band
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Protocols

Gel Filtration Markers Kit for Protein Molecular Weights 12,000-200,000 Da

Gel filtration chromatography is an established method for determining the size and molecular mass of proteins.

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