A8850
Auto-Activated Protein Kinase from bovine kidney
from bovine kidney, aqueous solution
Synonym(s):
AK
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About This Item
Specific activity:
~17,000 units/mg protein
Biological source:
bovine kidney
Quality Segment
biological source
bovine kidney
description
Minimum 50 ng protein/vial by Bradford
form
aqueous solution
specific activity
~17,000 units/mg protein
mol wt
36 kDa
shipped in
dry ice
storage temp.
−70°C
Biochem/physiol Actions
This enzyme phosphorylates and inactivates protein phosphatase 2A. The funtion appears to be related to the catalytic domain of p21-activated p65(PAK) protein kinase which is produced in apoptotic cells. AK is also involved in cytoskeleton organization and other signal transduction processes.
Physical form
50 ng per vial in 10 μl of 50 mM Tris-HCl, pH 7.0, containing 14 mM β-Mercaptoethanol, 1 mM benzamidine, 0.1 mM PMSF, 1 mM EDTA, 65% ethyleneglycol, and 0.1% Brij® 35.
Other Notes
One unit is the amount of enzyme that will incorporate 1 nmole of phosphoryl groups using myelin basic protein as a substrate per minute at pH 7 at 30 deg C.
Legal Information
Brij is a registered trademark of Croda International PLC
Storage Class
10 - Combustible liquids
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