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Merck
CN

A8917

Anti-Bovine IgG (whole molecule)−Peroxidase antibody produced in rabbit

IgG fraction of antiserum, buffered aqueous solution

Synonym(s):

Rabbit Anti-Bovine IgG (whole molecule)−HRP

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About This Item

UNSPSC Code:
12352203
NACRES:
NA.46
MDL number:
MFCD00162171
Conjugate:
peroxidase conjugate
Clone:
polyclonal
Application:
DB, ELISA (d), IHC (p)
Citations:
9

Key Documents

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biological source

rabbit

conjugate

peroxidase conjugate

antibody form

IgG fraction of antiserum

antibody product type

secondary antibodies

clone

polyclonal

form

buffered aqueous solution

species reactivity

bovine

technique(s)

direct ELISA: 1:20,000, dot blot: 1:80,000, immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:1,000

shipped in

dry ice

storage temp.

−20°C

target post-translational modification

unmodified

Quality Level

200

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General description

Immunoglobulin G (IgG) contributes to 10−20% of plasma protein and is regarded as one of the most predominant serum protein. It consists of four subclasses: IgG1, IgG2, IgG3 and IgG4. The IgG structure possesses four polypeptide chains containing two identical γ heavy (H) chains and two identical κ or λ light (L) chains of 50 kDa and 25 kDa, respectively.

Immunogen

purified bovine IgG

Application

Anti-Bovine IgG (whole molecule) Peroxidase antibody produced in rabbit has been used in:
  • Dot- enzyme-linked immunosorbent assay (ELISA)
  • immunoblotting
  • immunohistology

Rabbit Anti-Bovine IgG (whole molecule)-Peroxidase antibody has been used for western blot and ELISA. The antibody can also be used for dot blot (1:80,000) and immunohistochemistry (1:1,000).

Biochem/physiol Actions

Bovine IgGs are glycoprotein antibodies that regulate immune responses in herds. These antibodies inhibit TLR5 activation upon immunization with native H7 flagellin. Bovine IgG levels can be used for the detection of Johne′s disease and milk adulteration.

Physical form

Solution in 0.01 M phosphate buffered saline pH 7.4, containing 0.05% MIT

Preparation Note

Prepared by the two-step glutaraldehyde method described by Avrameas, S., et al., Scand. J. Immunol., 8, Suppl. 7, 7 (1978).

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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pictograms

Health hazard
GHS08

signalword

Danger

hcodes

H317,H334

Hazard Classifications

Resp. Sens. 1 - Skin Sens. 1

Storage Class

12 - Non Combustible Liquids

wgk

WGK 2

flash_point_f

Not applicable

flash_point_c

Not applicable

Regulatory Information

低风险生物材料
常规特殊物品
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Certificates of Analysis (COA)

Lot/Batch Number
0000474679
0000474679
0000425146
0000425146
0000403807

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Development of Rapid Diagnostic Kit for Identification of Hanwoo (Korean Native Cattle) Brand Meat by Detecting BIO-TAG
Baek KH, et al.
Korean journal for food science of animal resources, 34(3), 339-339 (2014)
Comparison of crude and excretory/secretory antigens for the diagnosis of Fasciola hepatica in sheep by western blotting.
Kara, M. and Kircali, F.
Turkish Journal of Veterinary and Animal Sciences, 28, 943-949 (2004)
Investigation of antigenic specificity against Cysticercus tenuicollis cyst fluid antigen in dogs experimentally infected with Taenia hydatigena
Kara M and Douganay A
Turkish Journal of Veterinary and Animal Sciences, 29, 835-840 (2005)
Ingrid Ieda Fernando de Souza et al.
The Journal of veterinary medical science, 81(1), 9-14 (2018-10-12)
Bovine tuberculosis (bTB) control programs generally rely on intradermal tuberculin tests for the antemortem diagnosis of Mycobacterium bovis infection in cattle, but these tests detect only a portion of the infected animals. The aim of the present study was to
George C Russell et al.
Journal of virological methods, 299, 114329-114329 (2021-10-16)
The minor capsid protein of ovine herpesvirus 2, identified as a potential antigen for serological testing, was over-expressed and purified to allow its assessment in ELISA. The corresponding gene sequence (OvHV-2 orf65, Ov65) was modified to incorporate epitope tags and
View All Related Papers

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Data Sheet - A8917

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