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A9205

Sigma-Aldrich

Bovine Serum Albumin solution

30% in saline, fatty acid free, aseptically filled

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Synonym(s):
Albumin solution from bovine serum, BSA solution
CAS Number:
9048-46-8
MDL number:
MFCD00130384
NACRES:
NA.27

biological source

bovine

Quality Level

200

sterility

aseptically filled

Assay

28-32% protein basis (biuret)

form

solution

purified by

cold ethanol fractionation

does not contain

stabilizer or preservative

origin

USA origin

concentration

28-32% protein (biuret)
30% in saline

free fatty acid content

≤0.01%

technique(s)

ELISA: suitable
electrophoresis: suitable
western blot: suitable

impurities

≤0.01% free fatty acid

UniProt accession no.

P02769

foreign activity

BT virus, none detected
VSV virus, none detected

storage temp.

2-8°C

Gene Information

bovine ... ALB(280717)

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General description

Bovine Serum Albumin (BSA) has three homologous domains, each subdivided it two domain (A and B). It is a α-helical globular protein and shares structural features with human serum albumin.

Application

Bovine Serum Albumin solution has been used:
  • as Dulbecco′s modified Eagle′s medium supplement for culturing HepG2 human liver cancer cells
  • as a component of hepatocyte recovery medium
  • as a component of fusion and activation buffers for in vitro reconstructed sheep embryos

Biochem/physiol Actions

Bovine Serum Albumin (BSA) is the major protein present in blood and has calcium binding sites and regulates metal homeostasis. It is a crucial component of the cell culture medium and promotes embryonic stem cells (hESC) differentiation. Due to its high solubility, low cost and purity it is widely used in cosmetic and pharmaceutical industry. BSA is used as a blocking agent used in enzyme linked immunosorbent assay (ELISA) for preventing non-specific binding of antigens and antibodies to the microtiter plates.

Preparation Note

Initial fractionation by cold alcohol precipitation

Storage Class Code

10 - Combustible liquids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Regulatory Information

含少量动物源组分生物产品

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