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A9205

Bovine Serum Albumin solution

Name: Bovine Serum Albumin solution
Brand: SIGMA

30% in saline, fatty acid free, aseptically filled

Synonym(s):

Albumin solution from bovine serum, BSA solution

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About This Item

CAS Number:

9048-46-8

UNSPSC Code:

12352202

NACRES:

NA.27

MDL number:

MFCD00130384

eCl@ss:

32160409

Biological source:

bovine

Assay:

28-32% protein basis (biuret)

Form:

solution

Technique(s):

ELISA: suitable, electrophoresis: suitable, western blot: suitable

Impurities:

≤0.01% free fatty acid

Concentration:

28-32% protein (biuret), 30% in saline

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biological source

bovine

Quality Level

200

sterility

aseptically filled

assay

28-32% protein basis (biuret)

form

solution

purified by

cold ethanol fractionation

does not contain

stabilizer or preservative

origin

USA origin

concentration

28-32% protein (biuret), 30% in saline

free fatty acid content

≤0.01%

technique(s)

ELISA: suitable, electrophoresis: suitable, western blot: suitable

impurities

≤0.01% free fatty acid

UniProt accession no.

P02769

foreign activity

BT virus, none detected, VSV virus, none detected

storage temp.

2-8°C

Gene Information

bovine ... ALB(280717)

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Related Categories

Albumins and Transport Proteins

Bovine Serum Albumin

Cell Culture & Analysis

Cell Culture Supplements & Reagents

Molecular Biology & Functional Genomics

General description

Bovine Serum Albumin (BSA) has three homologous domains, each subdivided it two domain (A and B). It is a α-helical globular protein and shares structural features with human serum albumin.

Application

Bovine Serum Albumin solution has been used:

as Dulbecco′s modified Eagle′s medium supplement for culturing HepG2 human liver cancer cells
as a component of hepatocyte recovery medium
as a component of fusion and activation buffers for in vitro reconstructed sheep embryos

Biochem/physiol Actions

Bovine Serum Albumin (BSA) is the major protein present in blood and has calcium binding sites and regulates metal homeostasis. It is a crucial component of the cell culture medium and promotes embryonic stem cells (hESC) differentiation. Due to its high solubility, low cost and purity it is widely used in cosmetic and pharmaceutical industry. BSA is used as a blocking agent used in enzyme linked immunosorbent assay (ELISA) for preventing non-specific binding of antigens and antibodies to the microtiter plates.

Preparation Note

Initial fractionation by cold alcohol precipitation

Storage Class

10 - Combustible liquids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)

Regulatory Information

监管及禁止进口产品

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Certificates of Analysis (COA)

Lot/Batch Number

0000520690

0000504183

0000504182

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Global Trade Item Number

SKU	GTIN
A9205-10ML	04061833401569
A9205-1L	04061838168757
A9205-50ML	04061833401576

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