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Merck
CN

A9325

L-Alanine 4-nitroanilide hydrochloride

protease substrate, ≥99% (TLC), powder

Synonym(s):

Ala-4-nitroanilide, H-Ala-pNA

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About This Item

Empirical Formula (Hill Notation):
C9H11N3O3 · HCl
CAS Number:
31796-55-1
Molecular Weight:
245.66
Beilstein:
6303731
EC Number:
250-811-0
MDL number:
MFCD00039088
UNSPSC Code:
12352204
eCl@ss:
32160406
PubChem Substance ID:
24891436
NACRES:
NA.83

Key Documents

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Product Name

L-Alanine 4-nitroanilide hydrochloride, protease substrate

Assay

≥99% (TLC)

form

powder

solubility

water: 50 mg/mL, clear to very slightly hazy

storage temp.

−20°C

SMILES string

Cl[H].C[C@H](N)C(=O)Nc1ccc(cc1)[N+]([O-])=O

InChI

1S/C9H11N3O3.ClH/c1-6(10)9(13)11-7-2-4-8(5-3-7)12(14)15;/h2-6H,10H2,1H3,(H,11,13);1H/t6-;/m0./s1

InChI key

YEXRLSXNWLNHQR-RGMNGODLSA-N

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Application

L-Alanine 4-nitroanilide hydrochloride has been used to determine the activity of aminopeptidase in the intestinal segments and small intestine (jejunum and ileum).

Biochem/physiol Actions

L-Alanine-4-nitroanilide is used as a substrate to determine alanine aminopeptidase (AAP) activity. It is mainly chosen as a substrate, due to its relative specificity, higher hydrolysis rate and better solubility.

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

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Certificates of Analysis (COA)

Lot/Batch Number
BCCN1153
BCCK5641
BCCD6991
BCBG9297V
BCBV4313

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Methods for Measuring Urinary Enzyme Activities
Urinary Enzymes, 99-145 (1992)
Effect of insoluble fiber supplementation applied at different ages on digestive organ weight and digestive enzymes of layer-strain poultry
Yokhana J S, et al.
Poultry Science, 95(3), 550-559 (2015)

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