Skip to Content
Merck
CN

Key Documents

View All Documentation

A9647

Bovine Serum Albumin

heat shock fraction, pH 7, ≥98%

Synonym(s):

Albumin bovine serum, BSA, Bovine albumin

Sign In to View Organizational & Contract Pricing.

Select a Size

Change View

About This Item

CAS Number:
9048-46-8
UNSPSC Code:
12352202
EC Number:
232-936-2
NACRES:
NA.27
MDL number:
MFCD00130384
eCl@ss:
32160409
Biological source:
bovine
Assay:
≥98%
Form:
lyophilized powder
Technique(s):
immunocytochemistry: suitable
Impurities:
≤5.0% Loss on drying
Technical Service
Need help? Our team of experienced scientists is here for you.
Let Us Assist

biological source

bovine

Quality Level

300

assay

≥98%

form

lyophilized powder

purified by

heat shock fractionation

origin

USA origin

technique(s)

immunocytochemistry: suitable

impurities

≤5.0% Loss on drying

loss

≤5%

pH

7

solubility

water: soluble (40 mg/ml)

UniProt accession no.

P02769

foreign activity

BT virus, none detected, VSV virus, none detected

storage temp.

2-8°C

Gene Information

bovine ... ALB(280717)

Looking for similar products? Visit Product Comparison Guide

Related Categories

General description

Bovine serum albumin is a protein whose key biological function is to regulate the colloidal osmotic pressure of blood. BSA is widely used in various studies and applications. Made up of 583 amino acids, BSA is a water-soluble protein with a calculated molecular weight of 66,430 Da. Six α-helices form three homologous domains of BSA. Depending on pH, it undergoes reversible conformational isomerization. The native structure of the protein becomes reactive and flexible on heating.

Application

Bovine Serum Albumin is used for many applications, including the following applications:
  • BSA has been used in cell culture, e.g. as a supplement in media prepared for culturing of oligodendrocytes.
  • BSA has been used for the dilution of primary antibody solution in immunohistochemistry procedures.
  • BSA has been used as a standard in the Bradford method of protein quantification.
  • BSA has been used in blocking and permeabilization buffer for immunofluorescence studies.

Biochem/physiol Actions

Certain conformational and primary-sequence epitopes of BSA are suspected allergens in human beef and milk allergies.

Preparation Note

Prepared using heat shock fractionation
Serum albumin may be referred to as Fraction V. This naming convention is taken from the original Cohn method of fractionating serum proteins using cold ethanol precipitation. Serum albumin was found in the fifth ethanol fraction using Cohn′s method. Since then, the term "Fraction V" has been used by some to describe serum albumin regardless of the method of preparation. Others have used this term to describe serum albumin purified by ethanol fractionation methods that have been highly modified since the original Cohn method was described. Sigma-Aldrich manufactures and distributes serum albumins purified from a variety of primary methods including the true Cohn fractionation method, modified ethanol fractionation methods, heat shock and chromatography. Additional purification steps may include crystallization or charcoal filtration.

Storage Class

11 - Combustible Solids

wgk

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)

Regulatory Information

监管及禁止进口产品

This item has

Choose from one of the most recent versions:

Certificates of Analysis (COA)

Lot/Batch Number
0000543522
0000543523
0000543524
0000543519
0000529015

Don't see the Right Version?

If you require a particular version, you can look up a specific certificate by the Lot or Batch number.

Search for a COA

Already Own This Product?

Find documentation for the products that you have recently purchased in the Document Library.

Visit the Document Library

Mohammad Bahrami et al.
Scientific reports, 9(1), 11739-11739 (2019-08-15)
Defining oocyte in vitro maturation (IVM) conditions allows for improved reproducibility and efficiency of bovine embryo production. IVM conditions for bovine oocytes have been extensively studied, but beneficial effects of individual supplements remain controversial. This study compared methods of cumulus
Characterization of an Exo-beta-1,3-Glucanase Produced by Pichia anomala Strain K, Antagonist of Botrytis cinerea on Apples.
Jijakli MH et al.
Phytopathology, 88, 335-335 (1998)
Down-regulation of seladin-1 increases BACE1 levels and activity through enhanced GGA3 depletion during apoptosis.
Sarajarvi T et al.
The Journal of Biological Chemistry, 284, 34433-34433 (2009)
E-cadherin, MMP-2, and MMP-9 as prognostic markers in penile cancer: analysis of 125 patients.
Campos RS et al.
Urology, 67, 797-797 (2006)
Bettina Gerstner et al.
Journal of neuroscience research, 87(9), 2078-2086 (2009-02-19)
Developing oligodendrocytes (pre-OLs) are highly vulnerable to hypoxic-ischemic injury and associated excitotoxicity and oxidative stress. 17beta-Estradiol plays an important role in the development and function of the CNS and is neuroprotective. The sudden drop in circulating estrogen after birth may
View All Related Papers

Articles

Colorimetric Detection of Western blot using 4-Chloro-1-Naphthol Solution Protocol

This solution is used as a substrate for detecting horseradish-peroxidase conjugates in Western blotting.

Fast Analysis of Monoclonal Antibodies Using Silica Monoliths Designed for Bioanalysis

Experience effective immobilization of protein A on wide-pore epoxy-modified silica monoliths for mAb separation, showcasing the efficiency of Chromolith® WP 300 columns in achieving faster and highly reproducible biomolecule separations.

Albumin in Cell Culture

Explore serum albumin's role in serum-free cell culture systems for biomanufacturing.

白蛋白在细胞培养中的应用

本页将全面阐述血清白蛋白和其他重要细胞培养成分如何影响用于单克隆抗体等外源蛋白生物制造的无血清细胞培养系统的性能，并介绍白蛋白的体外化学和生物化学过程。本页面将介绍白蛋白在体外化学和生物化学领域的应用。这些深度知识可能也适用于离体和组织工程学应用。

Protocols

Enzymatic Assay of Alcohol Dehydrogenase (EC 1.1.1.1)

To measure alcohol dehydrogenase activity, this assay uses β-nicotinamide adenine dinucleotide phosphate and a continuous spectrophotometric rate determination at 340 nm.

Immunohistochemistry Procedure

Use this protocol to for the entire immunohistochemistry (IHC) procedure through staining and visualization of specific antigens in paraffin-embedded tissue sections.

醇脱氢酶（EC 1.1.1.1）的酶学测定

在测定醇脱氢酶活性时，该测定使用β-烟酰胺腺嘌呤二核苷酸磷酸和在340nm处的连续分光光度法进行测定。

免疫组织化学操作步骤

本实验方案通过对石蜡包埋的组织切片中的特定抗原进行染色和成像，完成整个免疫组织化学（IHC）操作步骤。

Related Content

Preparation & Staining of 3D Printed Tissues & Scaffolds

Three-dimensional (3D) printing of biological tissue is rapidly becoming an integral part of tissue engineering.

Protein Research Guide

Find protein research tools to prepare, isolate, and analyze proteins. Organized by how to extract, protect, purify, enrich, modify, and quantify proteins.

Global Trade Item Number

SKUGTIN
A9647-500G04061835553730
A9647-10G04061835553716
A9647-100G04061835517657
A9647-5G04061833426005
A9647-1KG04061835553723
A9647-50G04061835511785

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Contact Technical Service