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Merck
CN

A9771

Sigma-Aldrich

Albumin–fluorescein isothiocyanate conjugate

protein bovine

Synonym(s):

FITC-albumin

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About This Item

MDL number:
MFCD00282182
UNSPSC Code:
12352204
eCl@ss:
42010201
NACRES:
NA.32

Key Documents

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biological source

protein from bovine

form

powder

extent of labeling

≥7 mol FITCmol albumin

technique(s)

ELISA: suitable

solubility

10 mM Tris pH 7.0: 10 mg/mL

application(s)

detection

storage temp.

2-8°C

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General description

Albumin makes up 60% of the plasma protein, which is produced by the liver. It regulates colloid osmotic pressure. Albumin is implicated in the transportation, distribution and metabolism of fatty acids, thyroid hormones and drugs. It reduces weight gain, blocks pulmonary edema, decreases ascites and lowers tissue edema.

Application

Albumin−fluorescein isothiocyanate conjugate has been used:
  • as a control to compare the intracellular delivery efficiency of proteins with or without carrier
  • for the fluorescent visualization of proteins and cells
  • as a blocking agent in ELISA
  • as a chemical for brain perfusion and fixation
  • for in vivo capillary–epithelial permeability experiment
  • for confocal microscopy of right ventricular (RV) vasculature in rats

Preparation Note

Prepared from crystallized and lyophilized albumin (A 4378).

Pictograms

Health hazard
GHS08

Signal Word

Danger

Hazard Statements

H317,H334

Hazard Classifications

Resp. Sens. 1 - Skin Sens. 1

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Regulatory Information

监管及禁止进口产品
This item has

Choose from one of the most recent versions:

Certificates of Analysis (COA)

Lot/Batch Number
0000462068
0000462067
0000462064
0000462066
0000462067

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  1. Which document(s) contains shelf-life or expiration date information for a given product?

    If available for a given product, the recommended re-test date or the expiration date can be found on the Certificate of Analysis.

  2. How do I get lot-specific information or a Certificate of Analysis?

    The lot specific COA document can be found by entering the lot number above under the "Documents" section.

  3. What are the excitation and emission maxima for Product A9771, Albumin-fluorescein isothiocyanate conjugate (FITC-albumin)?

    The excitation maximum is 495 nm and the emission maximum is 520 nm.

  4. How is the FITC bound to the albumin in Product A9771, Albumin-fluorescein isothiocyanate conjugate (FITC-albumin)?

    The FITC is bound through the epsilon amino group of the lysine amino acid residues. Each lot number will have a varying number of FITC molecules bound.

  5. Is there any free FITC present in Product A9771, Albumin-fluorescein isothiocyanate conjugate?

    We would not expect there to be free FITC. The final product is dialyzed against distilled water using dialysis tubing with a molecular weight cutoff of 12,000 prior to it being lyophilized.

  6. How do I find price and availability?

    There are several ways to find pricing and availability for our products. Once you log onto our website, you will find the price and availability displayed on the product detail page. You can contact any of our Customer Sales and Service offices to receive a quote.  USA customers:  1-800-325-3010 or view local office numbers.

  7. What is the Department of Transportation shipping information for this product?

    Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product. 

  8. My question is not addressed here, how can I contact Technical Service for assistance?

    Ask a Scientist here.

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Giridhar PV, et al.
Journal of Immunology, 23(7), 1600926-1600926 (2016)
George A Tanner et al.
American journal of physiology. Renal physiology, 296(6), F1269-F1278 (2009-04-03)
Many aspects of the glomerular filtration of macromolecules remain controversial, including the location of the major filtration barrier, the effects of electrical charge, and the reason the filtration barrier does not clog. We examined these issues in anesthetized Necturus maculosus
Georgette L Suidan et al.
PloS one, 3(8), e3037-e3037 (2008-08-30)
Disruption of the blood brain barrier (BBB) is a hallmark feature of immune-mediated neurological disorders as diverse as viral hemorrhagic fevers, cerebral malaria and acute hemorrhagic leukoencephalitis. Although current models hypothesize that immune cells promote vascular permeability in human disease
Philbert S Tsai et al.
The Journal of neuroscience : the official journal of the Society for Neuroscience, 29(46), 14553-14570 (2009-11-20)
It is well known that the density of neurons varies within the adult brain. In neocortex, this includes variations in neuronal density between different lamina as well as between different regions. Yet the concomitant variation of the microvessels is largely
Adhesion and Cellular Compatibility of Silicone-Based Skin Adhesives
Fischer SCL
Macromolecular Materials and Engineering, 302(5), 1600526-1600526 (2017)
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