AA0100
Ammonia Assay Kit
sufficient for 100 assays
Synonym(s):
Ammonia Quantitative Test Kit
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About This Item
NACRES:
NA.84
UNSPSC Code:
12352202
Application
Ammonia concentration was measured in serum and urine using the Sigma Ammonia Assay Kit in a study assessing expression of renal ammonia transporters in response to chromic metabolic acidosis.
This kit is for the quantitative, enzymatic determination of ammonia in food and biological samples. Ammonia reacts with α-ketoglutaric acid (KGA) and reduced nicotinamide adenine dinucleotide phosphate (NADPH) in the presence of L-glutamate dehydrogenase (GlDH) to form L-glutamate and oxidized nicotinamide adenine dinucleotide phosphate (NADP+). The decrease in absorbance at 340 nm, due to the oxidation of NADPH, is proportional to the ammonia concentration. L-Glutamate dehydrogenase reacts specifically with ammonia. The Ammonia Assay Kit may be used to determine ammonia concentrations in the range of 0.2-15 μg/ml.
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Kit Components Only
Product No.
Description
- Ammonia Assay Reagent 10 vial(s)
- L-Glutamate Dehydrogenase for Ammonia Assay 1 mL/vial
- Ammonia Standard Solution 1 mL/vial
signalword
Danger
hcodes
Hazard Classifications
Eye Irrit. 2 - Resp. Sens. 1
Storage Class
10 - Combustible liquids
flash_point_f
Not applicable
flash_point_c
Not applicable
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