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Merck
CN

AA0100

Ammonia Assay Kit

sufficient for 100 assays

Synonym(s):

Ammonia Quantitative Test Kit

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About This Item

NACRES:
NA.84
UNSPSC Code:
12352202
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usage

sufficient for 100 assays

Quality Level

storage temp.

2-8°C

Application

Ammonia concentration was measured in serum and urine using the Sigma Ammonia Assay Kit in a study assessing expression of renal ammonia transporters in response to chromic metabolic acidosis.
This kit is for the quantitative, enzymatic determination of ammonia in food and biological samples. Ammonia reacts with α-ketoglutaric acid (KGA) and reduced nicotinamide adenine dinucleotide phosphate (NADPH) in the presence of L-glutamate dehydrogenase (GlDH) to form L-glutamate and oxidized nicotinamide adenine dinucleotide phosphate (NADP+). The decrease in absorbance at 340 nm, due to the oxidation of NADPH, is proportional to the ammonia concentration. L-Glutamate dehydrogenase reacts specifically with ammonia. The Ammonia Assay Kit may be used to determine ammonia concentrations in the range of 0.2-15 μg/ml.


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Kit Components Only

Product No.
Description

  • Ammonia Assay Reagent 10 vial(s)

  • L-Glutamate Dehydrogenase for Ammonia Assay 1 mL/vial

  • Ammonia Standard Solution 1 mL/vial

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Danger

pictograms

Health hazard

hcodes

Hazard Classifications

Eye Irrit. 2 - Resp. Sens. 1

Storage Class

10 - Combustible liquids

flash_point_f

Not applicable

flash_point_c

Not applicable

Regulatory Information

监管及禁止进口产品

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Articles

Sigma article discusses tumor cell metabolic pathways, focusing on aerobic glycolysis and mitochondrial activity.

Fatty acid synthesis supports cancer cell proliferation, essential for membrane generation, protein modification, and bioenergetics.

本文介绍了增殖活性细胞为何需要碳源和氮源合成大分子。尽管大部分肿瘤细胞利用有氧糖酵解途径并分流线粒体氧化磷酸化代谢物,但许多肿瘤细胞表现出线粒体活性增加。

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Global Trade Item Number

SKUGTIN
AA0100-1KT04061833418369